Preimplantation development of in vitro-matured and in vitro-fertilized ovine zygotes: comparison between coculture on oviduct epithelial cell monolayers and culture under low oxygen atmosphere.
Andrew J. Watson,Patricia H. Watson,Deirdre Warnes,Simon K. Walker,David T. Armstrong,David T. Armstrong,Robert F. Seamark +6 more
TLDR
Two distinct culture environments are characterized, each capable of supporting the development of high frequencies of unselected IVMF zygotes to the blastocyst stage in vitro, and survival of the embryo under a reduced oxygen atmosphere is investigated.Abstract:
The roles of medium composition, serum source, embryo coculture, and culture under low O2 conditions on the development of in vitro-matured and in vitro-fertilized (IVMF) ovine zygotes were investigated in three separate experiments. In the first experiment, the proportion of cocultured IVMF zygotes developing to the blastocyst stage was significantly higher (38.0% vs. 3.5%; p < 0.05) than that of non-cocultured zygotes treated within three embryo culture media (TCM-199 + 10% fetal bovine serum [FBS]; bicarbonate-buffered, glucose-free synthetic oviduct fluid medium [mod-SOFM] + 10% FBS; and bicarbonate-buffered BSA-free Tyrode's salt solution [mod-TALP] + 10% FBS) under a 5% CO2 atmosphere in air. In a second experiment, a significantly higher (p < 0.05) proportion of cocultured zygotes placed in TCM-199 medium survived to the blastocyst stage (37.4% blastocysts vs. 23.4% in mod-SOFM). No significant effect of serum (FBS vs. human serum [HS]) was observed on embryonic development, but coculture was confirmed to exert a significant influence on development to the blastocyst stage. In the final experiment, survival of the embryo under a reduced oxygen (5% CO2:5% O2:90% N2) atmosphere was investigated. In contrast to results in the initial experiments, embryonic survival was significantly higher (p < 0.05) in the non-cocultured treatment groups (21.9% blastocysts vs. 0.4% for cocultured zygotes). Serum source also had a significant (p < 0.05) influence upon the development of non-cocultured zygotes: 32.3% of zygotes cultured with HS progressed to the blastocyst stage vs. 11.5% of zygotes cultured in FBS-supplemented medium. These results have characterized two distinct culture environments, each capable of supporting the development of high frequencies of unselected IVMF zygotes to the blastocyst stage in vitro.read more
Citations
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Follicle-oocyte atresia and temporal taphonomy in cold-stored domestic cat ovaries.
TL;DR: There is marked variability in the number and quality of follicle populations in cat ovaries, and a high percentage of full‐sized follicular oocytes are undergoing atresia at any given time, likely contributing to comparatively lower IVM/IVF success than in other species.
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Factors affecting the developmental competence of mouse oocytes grown in vitro: oxygen concentration.
John J. Eppig,Karen Wigglesworth +1 more
TL;DR: It was concluded that concentrations of O2 above 5% have a deleterious effect on oocyte development during the early stages of culture, and increasing the concentration on O2 during the later stages, in conjunction with other treatments to promote normal granulosa cell development and function and their interaction with the oocyte, may be critical to promotenormal oocytes development in vitro.
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Preservation of the Wild European Mouflon: The First Example of Genetic Management Using a Complete Program of Reproductive Biotechnologies
Grazyna Ptak,Grazyna Ptak,Michael Clinton,Barbara Barboni,Marco Muzzeddu,P. Cappai,Marian Tischner,Pasqualino Loi +7 more
TL;DR: The rescue of an endangered species, the European mouflon (Ovis orientalis musimon), is described for the first time by the application of an integrated package of reproductive biotechnologies.
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Bovine oviductal and embryonic insulin-like growth factor binding proteins: possible regulators of "embryotrophic" insulin-like growth factor circuits.
TL;DR: The results suggest that maternally derived IGF may be regulated by IGFBPs to support bovine preattachment development.
Journal ArticleDOI
Development of in vitro matured/fertilized bovine embryos in a chemically defined medium: influence of oxygen concentration in the gas atmosphere.
TL;DR: It is demonstrated that oxygen concentration critically affects embryonic development through zygotes to blastocysts, and suggest that around 5% 02 is optimal, and indicates that bovine zygote can be cultured up to the blastocyst stage using a chemically defined medium with rates similar to those of a conventional coculture system.
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