Preimplantation development of in vitro-matured and in vitro-fertilized ovine zygotes: comparison between coculture on oviduct epithelial cell monolayers and culture under low oxygen atmosphere.
Andrew J. Watson,Patricia H. Watson,Deirdre Warnes,Simon K. Walker,David T. Armstrong,David T. Armstrong,Robert F. Seamark +6 more
TLDR
Two distinct culture environments are characterized, each capable of supporting the development of high frequencies of unselected IVMF zygotes to the blastocyst stage in vitro, and survival of the embryo under a reduced oxygen atmosphere is investigated.Abstract:
The roles of medium composition, serum source, embryo coculture, and culture under low O2 conditions on the development of in vitro-matured and in vitro-fertilized (IVMF) ovine zygotes were investigated in three separate experiments. In the first experiment, the proportion of cocultured IVMF zygotes developing to the blastocyst stage was significantly higher (38.0% vs. 3.5%; p < 0.05) than that of non-cocultured zygotes treated within three embryo culture media (TCM-199 + 10% fetal bovine serum [FBS]; bicarbonate-buffered, glucose-free synthetic oviduct fluid medium [mod-SOFM] + 10% FBS; and bicarbonate-buffered BSA-free Tyrode's salt solution [mod-TALP] + 10% FBS) under a 5% CO2 atmosphere in air. In a second experiment, a significantly higher (p < 0.05) proportion of cocultured zygotes placed in TCM-199 medium survived to the blastocyst stage (37.4% blastocysts vs. 23.4% in mod-SOFM). No significant effect of serum (FBS vs. human serum [HS]) was observed on embryonic development, but coculture was confirmed to exert a significant influence on development to the blastocyst stage. In the final experiment, survival of the embryo under a reduced oxygen (5% CO2:5% O2:90% N2) atmosphere was investigated. In contrast to results in the initial experiments, embryonic survival was significantly higher (p < 0.05) in the non-cocultured treatment groups (21.9% blastocysts vs. 0.4% for cocultured zygotes). Serum source also had a significant (p < 0.05) influence upon the development of non-cocultured zygotes: 32.3% of zygotes cultured with HS progressed to the blastocyst stage vs. 11.5% of zygotes cultured in FBS-supplemented medium. These results have characterized two distinct culture environments, each capable of supporting the development of high frequencies of unselected IVMF zygotes to the blastocyst stage in vitro.read more
Citations
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Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings
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Oxidative stress in an assisted reproductive techniques setting.
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Influence of in vitro systems on embryo survival and fetal development in cattle.
TL;DR: Findings indicate that exposure to some in vitro environments during the first 7 days of life can profoundly influence fetal and placental development in cattle.
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Impact of Bovine Oocyte Maturation Media on Oocyte Transcript Levels, Blastocyst Development, Cell Number, and Apoptosis
Andrew J. Watson,P.A. De Sousa,Anita N. Caveney,L. C. Barcroft,David R. C. Natale,J Urquhart,Mark E. Westhusin +6 more
TL;DR: The results demonstrate that blastocysts derived from in vitro maturation, fertilization, and embryo culture protocols undergo apoptosis but that apoptotic levels are not greatly influenced by the oocyte maturation environment.
References
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Journal ArticleDOI
Developmental potentiality of embryos cultured under low oxygen tension with superoxide dismutase.
TL;DR: The results suggest that the low oxygen-SOD culture conditions in this study are closer to the in vivo intratubal environment than the conventional culture conditions and that the protection of embryos from superoxide anion radicals is a prerequisite for the acquisition of embryos in vitro showing a developmental potentiality equal to that of embryo in vivo.
Journal ArticleDOI
Primary culture of human fallopian tube epithelial cells and co-culture of early mouse pre-embryos.
TL;DR: Cell proliferation was enhanced by epidermal growth factor, but not by fibroblast growth factors, insulin, transferrin, estradiol‐17β, or progesterone, according to a monolayer culture system established for human fallopian tube epithelial cells.
Journal ArticleDOI
In vitro development of ovine embryos in CZB medium.
TL;DR: A 2x2 factorial treatment arrangement was used to examine the effects of glucose and ethylenediaminetetraacetic acid on in vitro embryo development, and neither EDTA nor glucose affected in vitro embryos development based on the nuclear counts.
Journal ArticleDOI
Culture of one-cell bovine embryos in explanted mouse oviduct and bovine oviductal epithelial cells
TL;DR: The explanted immature mouse oviduct supported by TCM-199 did not provide an environment adequate for development of one-cell bovine embryos to the blastocyst stage, and development was best supported by co-culture with Oviductal epithelial cells in TCM -199 medium.
Journal ArticleDOI
Culture of ovine embryos in the absence of bovine serum albumin.
TL;DR: The overall degree of embryonic development was more advanced in BSA-supplemented media, and the concentrations of USG and SOL that were used in this study may not have been optimal for ovine embryo culture.
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