Journal ArticleDOI
Protein-induced long lifetime luminescence of nonmetal probes.
Erki Enkvist,Angela Vaasa,Marje Kasari,Marie Kriisa,Taavi Ivan,Kadri Ligi,Gerda Raidaru,Asko Uri +7 more
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TLDR
The discovered phenomenon, protein-induced long lifetime luminescence of aromatic probes is very likely to occur with all PKs and many other proteins.Abstract:
Time-resolved luminometry-based assays have great potential for measurements in complicated biological solutions and living cells as the measured signal can be easily distinguished from nanosecond lifetime background fluorescence of organic compounds and autofluorescence of cells. In the present study we discovered that binding of a thiophene- or a selenophene-containing heteroaromatic moiety (luminescence donor) to the purine-binding pocket of a protein kinase (PK) induces long lifetime photoluminescence signal that is largely intensified through efficient energy transfer to a fluorescent dye present in close proximity to the luminescence donor. The developed ARC-Lum probes possessing 19-266 μs luminescence lifetime when associated with the target kinase can be used for determination of activity of basophilic PKs, characterization of inhibitors of PKs, and as cAMP sensors. An ARC-Lum probe was also used for the determination of kinetic parameters of inhibitor binding to the catalytic subunit of protein kinase A (PKAc). Effective real-time monitoring of the activation of PKA by Forskolin and the displacement of an ARC-Lum probe from its complex with PKA by inhibitor H89 was performed in live cells. The discovered phenomenon, protein-induced long lifetime luminescence of aromatic probes is very likely to occur with all PKs and many other proteins.read more
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Recent advances in purely organic phosphorescent materials
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Cell-Penetrating Peptides Transport Noncovalently Linked Thermally Activated Delayed Fluorescence Nanoparticles for Time-Resolved Luminescence Imaging
TL;DR: These findings greatly expanded the applications of cell-penetrating peptides for delivery of molecules and NPs by only noncovalent interactions, which were more flexible and easier than covalent modifications.
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Photoactivity and optical applications of organic materials containing selenium and tellurium
TL;DR: Incorporating selenium or tellurium into photoactive species imparts new photophysical properties that may be exploited in materials applications.
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Highly Efficient Aggregation-Induced Red-Emissive Organic Thermally Activated Delayed Fluorescence Materials with Prolonged Fluorescence Lifetime for Time-Resolved Luminescence Bioimaging.
Sujie Qi,Sangin Kim,Van-Nghia Nguyen,Van-Nghia Nguyen,Youngmee Kim,Guangle Niu,Gyoungmi Kim,Sung Jin Kim,Sungnam Park,Juyoung Yoon +9 more
TL;DR: Three donor-acceptor-type TADF molecules are reported as promising candidates for time-resolved fluorescence imaging, which are engineered by direct connection of electron-donating moieties (phenoxazine or phenothiazine) and an electron- acceptor 1,8-naphthalimide (NI).
References
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Zwischenmolekulare Energiewanderung und Fluoreszenz
TL;DR: In this article, a quantenmechanische behandlung des Ubergangs von Elektronenanregungsenergie zwischen gleichartigen Molekulen in Losung gegeben.
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A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays.
TL;DR: A screening window coefficient, called "Z- factor," is defined, which is reflective of both the assay signal dynamic range and the data variation associated with the signal measurements, and therefore is suitable for assay quality assessment.
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Taking advantage of luminescent lanthanide ions
TL;DR: This critical review has been tailored for a broad audience of chemists, biochemists and materials scientists; the basics of lanthanide photophysics are highlighted together with the synthetic strategies used to insert these ions into mono- and polymetallic molecular edifices.
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Protein kinases--the major drug targets of the twenty-first century?
TL;DR: A personal view of some of the most important advances that have shaped this field of protein kinases, after G-protein-coupled receptors.
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The nuts and bolts of AGC protein kinases
TL;DR: The AGC kinase subfamily of protein kinases contains 60 members, including PKA, PKG and PKC, and their mutation and/or dysregulation contributes to the pathogenesis of many human diseases, including cancer and diabetes.