Journal ArticleDOI
Proteomic analysis of protein oxidation in Alzheimer's disease brain.
TLDR
The results suggest that the balance of protein oxidation and degradation is altered in AD, and this method can be applied to study oxidative changes of individual proteins in brain.Abstract:
There is a growing body of evidence that oxidative stress plays a major role in Alzheimer's disease (AD) pathogenesis. Identification of oxidatively altered proteins in AD is important for understanding the relationship between protein oxidation, protein aggregation and neurodegeneration. In this communication, we report a method that can be applied to study oxidative changes of individual proteins in brain. In order to analyze protein oxidation by detection of protein-bound carbonyls, cytosolic protein extracts were derivatized with 2,4-dinitrophenylhydrazine (DNPH) and then separated by two-dimensional (2-D) gel electrophoresis. After electrotransfer to polyvinylidene difluoride (PVDF) membranes, proteins were first stained with Sypro Ruby protein stain, and then the oxidized proteins were detected with anti-dinitrophenyl (DNP) antibody. About 150 proteins and more than 100 oxidized proteins were detected and quantified in both AD and control cases by 2-D image analysis. The amount of protein-bound carbonyls was decreased for six and increased for one protein in AD. The amount of protein was increased for three proteins in AD. Furthermore, the degree of oxidation was calculated as the ratio of protein-bound carbonyls to the total amount of an individual protein. Two proteins showed a significant decrease in the degree of oxidation in AD. Our results suggest that the balance of protein oxidation and degradation is altered in AD.read more
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Journal ArticleDOI
Protein carbonyl groups as biomarkers of oxidative stress.
TL;DR: The usage of protein CO groups as biomarkers of oxidative stress has some advantages in comparison with the measurement of other oxidation products because of the relative early formation and the relative stability of carbonylated proteins.
Journal ArticleDOI
GFAP in Health and Disease
Jinte Middeldorp,Elly M. Hol +1 more
TL;DR: The versatility of the GFAP cytoskeletal network from gene to function with a focus on astrocytes during human brain development, aging and disease is discussed.
Journal ArticleDOI
ROS production and protein oxidation as a novel mechanism for seed dormancy alleviation.
Krystyna Oracz,Hayat El-Maarouf Bouteau,Jill M. Farrant,Keren Cooper,Maya Belghazi,Claudette Job,Dominique Job,F. Corbineau,Christophe Bailly +8 more
TL;DR: In this paper, the role of reactive oxygen species (ROS) in after-ripening of sunflower seeds was investigated at the cellular level by electron microscopy, concomitantly with lipid peroxidation and oxidation of specific embryo proteins.
Journal ArticleDOI
Proteins as biomarkers of oxidative/nitrosative stress in diseases: The contribution of redox proteomics
Isabella Dalle-Donne,Andrea Scaloni,Daniela Giustarini,Eleonora Cavarra,Gianluca Tell,Giuseppe Lungarella,Roberto Colombo,Ranieri Rossi,Aldo Milzani +8 more
TL;DR: The study of oxidative/nitrosative modifications, investigated by redox proteomics, is contributing to establish a relationship between pathological hallmarks of disease and protein structural and functional abnormalities, enabling early detection of diseases.
Journal ArticleDOI
Two-dimensional gel electrophoresis in proteomics: Past, present and future.
TL;DR: A historical perspective is made, starting from the days where two-dimensional gels were used and the word proteomics did not even exist to a description of the now well-known limitations of two- dimensional gels in proteomics.