Journal ArticleDOI
Purification and Characterization of Pectin Lyase Produced by Aspergillus terricola and its Application in Retting of Natural Fibers
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TLDR
An indigenously isolatedfungal strain identified as Aspergillus terricola with assigned fungal strain number MTCC 7588 has been used as source for pectin lyase production and the retting ability of the purified pect in lyase for natural fibers has been demonstrated for the first time.Abstract:
An indigenously isolated fungal strain identified as Aspergillus terricola with assigned fungal strain number MTCC 7588 has been used as source for pectin lyase production. The extracellular pectin lyase was purified to homogeneity from the culture filtrate of A. terricola by ion exchange and gel filtration chromatography. The determined molecular weight was 35 ± 01 kDa. The K
m and k
cat (turnover) values of the purified enzyme at 37 °C using citrus pectin as the substrate were found to be 1.0 mg/ml and 110.0 s−1, respectively. The pH and temperature optima of the enzyme were 8.0 and 50 °C, respectively. The retting ability of the purified pectin lyase for natural fibers viz. Cannabis sativa and Linum usitatissimum has been demonstrated for the first time.read more
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Microbial pectinases: an ecofriendly tool of nature for industries
TL;DR: Pectinases are the growing enzymes of biotechnological sector, showing gradual increase in their market, and enzymatic catalysis is preferred over other chemical methods, since it is more specific, less aggressive and saves energy.
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Alkaline pectinases: A review
Pooja Kohli,Reena Gupta +1 more
TL;DR: In this paper, a review of the molecular determinants of pectin utilization, mechanisms driving catabolite selectivity and industrial applicability is presented, which provides structural understanding of the Pectinase topology and catalytic machinery.
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Biotechnological Potential of Agro-Industrial Wastes as a Carbon Source to Thermostable Polygalacturonase Production in Aspergillus niveus
Alexandre Maller,André Damasio,Tony Marcio da Silva,João Atílio Jorge,Héctor Francisco Terenzi,Maria de Lourdes Teixeira de Moraes Polizeli +5 more
TL;DR: The properties of this enzyme, produced on inexpensive fermentation substrates, were interesting and suggested several biotechnological applications.
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Pectinase Production from Schizophyllum commune Through Central Composite Design Using Citrus Waste and Its Immobilization for Industrial Exploitation
TL;DR: In this paper, a pectin enzyme from Schizophyllum commune using the mosambi (sweet lime) fruit peels as substrate in solid state fermentation was investigated.
References
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
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Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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Microbiology : A Laboratory Manual
TL;DR: In this article, basic laboratory techniques for isolation, cultivation, and cultural characterisation of microorganisms are described, including basic techniques for isolating, culturing, and characterizing microorganisms.