Journal ArticleDOI
Purification and properties of brain alkaline phosphatase.
TLDR
The homogeneous enzyme is shown to be a sialoglycoprotein in nature and shows pyridoxal phosphate phosphatase activity along with p‐nitrophenylphosphate phosphate activity, which behave as mutual alternate competitive substrates.Abstract:
— Alkaline phosphatase from sheep brain has been purified to homogeneity. The method includes butanol extraction, fractional ethanol precipitation, ion-exchange chromatography on DEAE-cellulose, and on DEAE-Sephadex followed by Sephadex G-200 filtration. By these steps, the enzyme is purified 22,920-fold with 15% recovery. The homogeneous enzyme is shown to be a sialoglycoprotein in nature. Neuraminidase treatment reduces the electrophoretic mobility of the enzyme. The enzyme shows pyridoxal phosphate phosphatase activity along with p-nitrophenylphosphate phosphatase activity. Both these compounds behave as mutual alternate competitive substrates. The general properties of the enzyme are described.read more
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Piperine modulates permeability characteristics of intestine by inducing alterations in membrane dynamics: influence on brush border membrane fluidity, ultrastructure and enzyme kinetics.
A. Khajuria,N. Thusu,U. Zutshi +2 more
TL;DR: It is suggested that piperine may be inducing alterations in membrane dynamics and permeation characteristics, along with induction in the synthesis of proteins associated with cytoskeletal function, resulting in an increase in the small intestine absorptive surface, thus assisting efficient permeation through the epithelial barrier.
Journal ArticleDOI
Butyrate and glucose metabolism by colonocytes in experimental colitis in mice.
M S Ahmad,Supriya Krishnan,Balakrishnan S. Ramakrishna,Minnie M. Mathan,Anna B. Pulimood,Sreekant Murthy +5 more
TL;DR: In this paper, butyrate metabolism was investigated in experimental colitis in mice, and colitis was induced in Swiss outbred white mice by oral administration of 4% dextran sulphate sodium (DSS), and colocytes isolated from colitic and normal control mice were incubated with [ 14 C]butyrate or glucose.
Journal ArticleDOI
Alginate coated chitosan core-shell nanoparticles for efficient oral delivery of naringenin in diabetic animals-An in vitro and in vivo approach.
TL;DR: Histopathology and several blood parameters indicated that oral administrations of nanoparticles were free from toxicity and suggested that polymeric formulations were quite effective for oral delivery of the flavonoid as a therapeutic agent in the treatment of dyslipidemia, hyperglycemia and haemoglobin iron-mediated oxidative stress in type 1 diabetic model.
Journal ArticleDOI
Oxidative stress in the development of liver cirrhosis: a comparison of two different experimental models.
Sathish Kumar Natarajan,Simmy Thomas,Prabhu Ramamoorthy,Jayasree Basivireddy,Anna B. Pulimood,Anup Ramachandran,Kunissery A. Balasubramanian +6 more
TL;DR: This study compares oxidative stress in the liver induced by these compounds at different stages of cirrhosis development in rats.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Colorimetric Method for Determination of Sugars and Related Substances
TL;DR: In this article, a method was developed to determine submicro amounts of sugars and related substances using a phenol-sulfuric acid reaction, which is useful for the determination of the composition of polysaccharides and their methyl derivatives.
Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
Journal ArticleDOI
Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.