Book ChapterDOI
Quantitation of protein.
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TLDR
This chapter discusses various methods of estimating protein concentration as defined by the difference in energy between the orbital of the unexcited electron and a higher energy orbital.Abstract:
Publisher Summary This chapter discusses various methods of estimating protein concentration. Absorption spectroscopy involves the absorption of a photon by an electron. Only those photons with a certain energy level can be absorbed as defined by the difference in energy between the orbital of the unexcited electron and a higher energy orbital. The peptide bond absorbs photons below 210 nm. Because of the large number of peptide bonds in a protein, this is a highly sensitive area of the protein spectrum. Although protein conformation and some absorption by tryptophan and tyrosine residues occurs in this region, less variability between proteins is observed than at 280 nm. There is a need to avoid storing buffers in plastic containers because some plastics leach plasticizers, which absorb at ultraviolet (UV) wavelengths. Detergents can also be troublesome because many absorb UV light. If the buffer or protein solution is cold, the outside of the cuvette may need to be wiped between each reading with a lint-free wiper and the readings should be made quickly after placing the cold solution into the cuvette, because atmospheric moisture may condense on the outside of the cuvette producing an erroneously high reading.read more
Citations
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Journal ArticleDOI
Characterization and tissue-specific expression of the two glutamate dehydrogenase cdnas in pacific white shrimp, litopenaeus vannamei
TL;DR: The results provide baseline data in physiological expressions of GDH and Na+-K+ ATPase for osmoregulation in L.vannamei and offers a practical guideline for the analysis of gene expression in shrimp.
Journal ArticleDOI
Detection of crosslinks within and between proteins by LC-MALDI-TOFTOF and the software FINDX to reduce the MSMS-data to acquire for validation.
Christopher A. G. Söderberg,Wietske Lambert,Sven Kjellström,Alena Wiegandt,Ragna Peterson Wulff,Cecilia Månsson,Gudrun Rutsdottir,Cecilia Emanuelsson +7 more
TL;DR: The decoupling of MS and MSMS and the peptide sorting with FINDX based on MS-data has the advantage that MSMS can be restricted to and focused on crosslinks of Type 2, which are of highest biological interest but often lowest in abundance.
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Trace element contamination in the Guadalquivir River Estuary ten years after the Aznalcóllar mine spill.
TL;DR: A significant decline of Zn in sediments and clams was observed compared to levels reported following the spill, so the estuarine ecosystem has recovered, however, the concentrations of some elements in S. plana were still higher than those of heavily contaminated regions.
Journal ArticleDOI
Pyrogallol red-molybdate: A reversible, metal chelate stain for detection of proteins immobilized on membrane supports
TL;DR: Certain metal complexes selectively interact with proteins immobilized on solid‐phase membrane supports to form brightly colored products, and the triazine dye‐ferrous complexes and the ferrocyanide‐ferric complexes provide the most sensitive detection of proteins immobilization on membranes.
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Characterization and pharmacological analysis of two adipokinetic hormone receptor variants of the tsetse fly, Glossina morsitans morsitans.
Jelle Caers,Tom Janssen,Liesbeth Van Rompay,Valérie Broeckx,Jan Van Den Abbeele,Gerd Gäde,Liliane Schoofs,Isabel Beets +7 more
TL;DR: Gene expression profiles suggest a function for AKH signaling in regulating Glossina energy metabolism, where AKH peptides are released from the corpora cardiaca and activate receptors mainly expressed in the fat body.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Book
Methods of Enzymatic Analysis
TL;DR: Methods of enzymatic analysis, Methods of enzymes analysis, the authors, Methods of enzyme analysis, enzymatics, methods of enzymes, and methods of analysis, method of enzymes.
Journal ArticleDOI
A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples
TL;DR: The original Lowry method of protein determination has been modified by the addition of sodium dodecyl sulfate in the alkali reagent and an increase in the amount of copper tartrate reagent to be used with membrane and lipoprotein preparations without prior solubilization or lipid extraction.