Book ChapterDOI
Quantitation of protein.
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TLDR
This chapter discusses various methods of estimating protein concentration as defined by the difference in energy between the orbital of the unexcited electron and a higher energy orbital.Abstract:
Publisher Summary This chapter discusses various methods of estimating protein concentration. Absorption spectroscopy involves the absorption of a photon by an electron. Only those photons with a certain energy level can be absorbed as defined by the difference in energy between the orbital of the unexcited electron and a higher energy orbital. The peptide bond absorbs photons below 210 nm. Because of the large number of peptide bonds in a protein, this is a highly sensitive area of the protein spectrum. Although protein conformation and some absorption by tryptophan and tyrosine residues occurs in this region, less variability between proteins is observed than at 280 nm. There is a need to avoid storing buffers in plastic containers because some plastics leach plasticizers, which absorb at ultraviolet (UV) wavelengths. Detergents can also be troublesome because many absorb UV light. If the buffer or protein solution is cold, the outside of the cuvette may need to be wiped between each reading with a lint-free wiper and the readings should be made quickly after placing the cold solution into the cuvette, because atmospheric moisture may condense on the outside of the cuvette producing an erroneously high reading.read more
Citations
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Journal ArticleDOI
The interaction of Naphthol Yellow S (NYS) with pepsin: Insights from spectroscopic to molecular dynamics studies.
TL;DR: The experimental results from fluorescence spectroscopy showed that the changes in pepsin's tertiary structure were caused by NYS binding, and NYS could be considered as an inhibitor with adverse effects on pepingin structure and function.
Journal ArticleDOI
Temperature‐Sensitive Microcapsules Containing Lactoferrin and Their Action Against Carnobacterium viridans on Bologna
Anas A. Al-Nabulsi,Jung H. Han,Zhiqiang Liu,Evangelina T. Rodrigues-Vieira,Richard A. Holley +4 more
TL;DR: In this article, paste-like microcapsules were prepared as water-in-oil (W 1 /O) emulsions from mixtures of 20% w/v LF in distilled water, 20% W/V LF in 3% w /v sodium lactate or in 20 mM sodium bicarbonate, which were emulsified with an oil mixture of 22% butter fat plus 78% corn oil and 0.1% polyglycerol polyricinoleate.
Journal ArticleDOI
Reconstitution and Characterization of a Calmodulin-Stimulated Ca-Pumping ATPase Purified from Brassica oleracea L.
Per Askerlund,David E. Evans +1 more
TL;DR: Sucrose gradient centrifugation suggests that the endoplasmic reticulum is the major cellular location of calmodulin-stimulated Ca(2+)-pumping ATPase in Brassica oleracea inflorescences.
Journal ArticleDOI
Comparative analysis of the proximate and elemental composition of the blue crab Callinectes sapidus, the warty crab Eriphia verrucosa, and the edible crab Cancer pagurus
Maurizio Zotti,Laura Del Coco,Sandra Angelica De Pascali,Danilo Migoni,Salvatrice Vizzini,Giorgio Mancinelli,Francesco Paolo Fanizzi +6 more
TL;DR: The proximate composition and element contents of claw muscle tissue of Atlantic blue crabs were compared with the native warty crab and the commercially edible crab to profile the chemical characteristics and nutritive value of the three crab species.
Journal ArticleDOI
Protease-Catalyzed Co-Oligomerizations of l-Leucine Ethyl Ester with l-Glutamic Acid Diethyl Ester: Sequence and Chain Length Distributions
TL;DR: In this paper, four proteases (papain, bromelain, α-chymotrypsin and protease SG) were used to catalyze co-oligomerizations of l-leucine ethyl ester (l-Et-Leu) with diethyl-l-glutamate (l-(Et)2-Glu).
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Book
Methods of Enzymatic Analysis
TL;DR: Methods of enzymatic analysis, Methods of enzymes analysis, the authors, Methods of enzyme analysis, enzymatics, methods of enzymes, and methods of analysis, method of enzymes.
Journal ArticleDOI
A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples
TL;DR: The original Lowry method of protein determination has been modified by the addition of sodium dodecyl sulfate in the alkali reagent and an increase in the amount of copper tartrate reagent to be used with membrane and lipoprotein preparations without prior solubilization or lipid extraction.