Book ChapterDOI
Quantitation of protein.
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This chapter discusses various methods of estimating protein concentration as defined by the difference in energy between the orbital of the unexcited electron and a higher energy orbital.Abstract:
Publisher Summary This chapter discusses various methods of estimating protein concentration. Absorption spectroscopy involves the absorption of a photon by an electron. Only those photons with a certain energy level can be absorbed as defined by the difference in energy between the orbital of the unexcited electron and a higher energy orbital. The peptide bond absorbs photons below 210 nm. Because of the large number of peptide bonds in a protein, this is a highly sensitive area of the protein spectrum. Although protein conformation and some absorption by tryptophan and tyrosine residues occurs in this region, less variability between proteins is observed than at 280 nm. There is a need to avoid storing buffers in plastic containers because some plastics leach plasticizers, which absorb at ultraviolet (UV) wavelengths. Detergents can also be troublesome because many absorb UV light. If the buffer or protein solution is cold, the outside of the cuvette may need to be wiped between each reading with a lint-free wiper and the readings should be made quickly after placing the cold solution into the cuvette, because atmospheric moisture may condense on the outside of the cuvette producing an erroneously high reading.read more
Citations
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A 17-kDa Nicotiana tabacum cell-wall peptide acts as an in-vitro inhibitor of the cell-wall isoform of acid invertase
TL;DR: P17 has been purified to homogeneity and its N-terminus has been sequenced, revealing no similarity to other known protein sequences, and possible physiological roles of p17 are discussed.
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Microbial reduction of Fe(III) in hematite nanoparticles by Geobacter sulfurreducens.
TL;DR: Surface area-normalized iron reduction rates were higher than those reported in previous studies, which may be due to the sequestration of Fe(II) through formation of vivianite.
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Molecular cloning and characterization of amylase from soil metagenomic library derived from Northwestern Himalayas
TL;DR: The ability of pAMY to work at low temperature is unique for amylases reported so far from microbes of cultured and uncultured division.
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Pasteurella multocida- and Pasteurella haemolytica-ghosts: new vaccine candidates.
J. Marchart,G. Dropmann,Sonja Lechleitner,T. Schlapp,Gerhard Wanner,Michael P. Szostak,Werner Lubitz +6 more
TL;DR: Ghosts of P. multocida and P. haemolytica are suggested as new vaccine candidates for immunization of rabbits and mice because of dose-dependent protection against homologous challenge and the number of proteins in whole cell protein extracts recognized by the sera constantly increased.
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Purification and characterization of a novel restricted antigen expressed by normal and transformed human colonic epithelium
Bruno Catimel,Gerd Ritter,Sydney Welt,Lloyd J. Old,Leonard Cohen,Maureen Nerrie,S.J. White,Joan K. Heath,B. Demediuk,Teresa Domagala,Fook-Thean Lee,Andrew M. Scott,Guo-Fen Tu,Hong Ji,Robert L. Moritz,Richard J. Simpson,Antony W. Burgess,E.C. Nice +17 more
TL;DR: A cell surface antigen that is expressed by normal and 95% of transformed colonic epithelium and is recognized by the monoclonal antibody A33 has been purified to homogeneity from the human colonic carcinoma cell line LIM1215.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Book
Methods of Enzymatic Analysis
TL;DR: Methods of enzymatic analysis, Methods of enzymes analysis, the authors, Methods of enzyme analysis, enzymatics, methods of enzymes, and methods of analysis, method of enzymes.
Journal ArticleDOI
A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples
TL;DR: The original Lowry method of protein determination has been modified by the addition of sodium dodecyl sulfate in the alkali reagent and an increase in the amount of copper tartrate reagent to be used with membrane and lipoprotein preparations without prior solubilization or lipid extraction.