Book ChapterDOI
Quantitation of protein.
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TLDR
This chapter discusses various methods of estimating protein concentration as defined by the difference in energy between the orbital of the unexcited electron and a higher energy orbital.Abstract:
Publisher Summary This chapter discusses various methods of estimating protein concentration. Absorption spectroscopy involves the absorption of a photon by an electron. Only those photons with a certain energy level can be absorbed as defined by the difference in energy between the orbital of the unexcited electron and a higher energy orbital. The peptide bond absorbs photons below 210 nm. Because of the large number of peptide bonds in a protein, this is a highly sensitive area of the protein spectrum. Although protein conformation and some absorption by tryptophan and tyrosine residues occurs in this region, less variability between proteins is observed than at 280 nm. There is a need to avoid storing buffers in plastic containers because some plastics leach plasticizers, which absorb at ultraviolet (UV) wavelengths. Detergents can also be troublesome because many absorb UV light. If the buffer or protein solution is cold, the outside of the cuvette may need to be wiped between each reading with a lint-free wiper and the readings should be made quickly after placing the cold solution into the cuvette, because atmospheric moisture may condense on the outside of the cuvette producing an erroneously high reading.read more
Citations
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Journal ArticleDOI
Copper Deficiency Induces the Upregulation of the Copper Chaperone for Cu/Zn Superoxide Dismutase in Weanling Male Rats
TL;DR: The possibility that the expression level of CCS may reflect copper status and thus serve as a useful marker of copper nutriture is explored and CCS protein level is reported as a novel marker for assessing copper status.
Journal ArticleDOI
Mechanism of malarial haem detoxification inhibition by chloroquine.
Amit V. Pandey,Himani Bisht,Vinod Babbarwal,Jaya Srivastava,Kailash C. Pandey,Virander S. Chauhan +5 more
TL;DR: The results indicate that chloroquine may be acting by inhibiting haem detoxification/binding to Pfhrp-2, and the higher affinity ofchloroquine for haem than PfhrP-2 suggests a possible mechanism of action for chlorquine; it may remove the haem bound to Pf hrps2 and form a complex that is toxic to the parasite.
Journal ArticleDOI
Investigation of interactions between poly-l-lysine-coated boron nitride nanotubes and C2C12 cells: up-take, cytocompatibility, and differentiation
Gianni Ciofani,Leonardo Ricotti,Serena Danti,Stefania Moscato,Claudia Nesti,Delfo D'Alessandro,Dinuccio Dinucci,Federica Chiellini,Andrea Pietrabissa,Mario Petrini,Arianna Menciassi +10 more
TL;DR: It is demonstrated that BNNTs are highly internalized by C2C12 cells, with neither adversely affecting C2 C12 myoblast viability nor significantly interfering with myotube formation.
Journal ArticleDOI
Protein fouling resistant membrane prepared by amphiphilic pegylated polyethersulfone
TL;DR: It was found that the PES-g-PEGMA membranes with higher surface coverage of PEG segments displayed stronger antibiofouling property, which was acquired due to covalent bonding interactions between hydrophilic PEGMA side chains and PES main chains.
Journal ArticleDOI
Dried plum polyphenols attenuate the detrimental effects of TNF-α on osteoblast function coincident with up-regulation of Runx2, Osterix and IGF-I
TL;DR: It is concluded that dried plum polyphenols enhance osteoblast activity and function by up-regulating Runx2, Osterix and IGF-I and increasing lysyl oxidase expression, and at the same time attenuate osteoclastogenesis signaling.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Book
Methods of Enzymatic Analysis
TL;DR: Methods of enzymatic analysis, Methods of enzymes analysis, the authors, Methods of enzyme analysis, enzymatics, methods of enzymes, and methods of analysis, method of enzymes.
Journal ArticleDOI
A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples
TL;DR: The original Lowry method of protein determination has been modified by the addition of sodium dodecyl sulfate in the alkali reagent and an increase in the amount of copper tartrate reagent to be used with membrane and lipoprotein preparations without prior solubilization or lipid extraction.