Book ChapterDOI
Quantitation of protein.
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TLDR
This chapter discusses various methods of estimating protein concentration as defined by the difference in energy between the orbital of the unexcited electron and a higher energy orbital.Abstract:
Publisher Summary This chapter discusses various methods of estimating protein concentration. Absorption spectroscopy involves the absorption of a photon by an electron. Only those photons with a certain energy level can be absorbed as defined by the difference in energy between the orbital of the unexcited electron and a higher energy orbital. The peptide bond absorbs photons below 210 nm. Because of the large number of peptide bonds in a protein, this is a highly sensitive area of the protein spectrum. Although protein conformation and some absorption by tryptophan and tyrosine residues occurs in this region, less variability between proteins is observed than at 280 nm. There is a need to avoid storing buffers in plastic containers because some plastics leach plasticizers, which absorb at ultraviolet (UV) wavelengths. Detergents can also be troublesome because many absorb UV light. If the buffer or protein solution is cold, the outside of the cuvette may need to be wiped between each reading with a lint-free wiper and the readings should be made quickly after placing the cold solution into the cuvette, because atmospheric moisture may condense on the outside of the cuvette producing an erroneously high reading.read more
Citations
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Journal ArticleDOI
Anticancer effects of saponin and saponin–phospholipid complex of Panax notoginseng grown in Vietnam
Thu Dang Kim,Hai Nguyen Thanh,Duong Nguyen Thuy,Loi Vu Duc,Thu Vu Thi,Hung Vu Manh,Patcharee Boonsiri,Tung Bui Thanh +7 more
TL;DR: In this article, the authors evaluated the antitumor activity both in vitro and in vivo of the saponin-phospholipid complex of Panax notoginseng.
Journal ArticleDOI
Purification and characterization of the single-strand-specific and guanylic-acid-preferential deoxyribonuclease activity of the extracellular nuclease from Basidiobolus haptosporus.
Neelam A. Desai,Vepatu Shankar +1 more
TL;DR: The absence of 5'dCMP as a hydrolytic product, coupled with the resistance of (dC)10 and deoxyribodinucleoside monophosphates having cytosine either at the 3' or the 5' end, indicates that C-linkages are resistant to cleavage by nuclease Bh1.
Journal ArticleDOI
The occurrence of phenol oxidase activity in female Trichuris suis.
Raymond H. Fetterer,Hill De +1 more
TL;DR: The results suggest that an enzyme of the phenol oxidase type is present in female worms but is probably inactive because of low oxygen tensions in the swine colon.
Journal ArticleDOI
DnaK prevents human insulin amyloid fiber formation on hydrophobic surfaces.
Thomas Ballet,Thomas Ballet,Franz Brukert,Paolo Mangiagalli,Christophe Bureau,Laurence Boulangé,Laurent Nault,Thomas Perret,Marianne Weidenhaupt +8 more
TL;DR: The peptide LVEALYL, derived from the insulin B chain, is known to promote fast aggregation in a concentration- and pH-dependent manner in solution and it is shown that it shortens the lag phase for insulin aggregation on hydrophobic surfaces, which indicates that the peptide and the chaperone might compete for a common site during the process of insulin aggregation.
Journal ArticleDOI
Effect of Gibberellin on Growth, Protein Secretion, and Starch Accumulation in Maize Endosperm Suspension Cells
Heping Cao,Jack C. Shannon +1 more
TL;DR: It is suggested that GA does not favor starch accumulation in the cell cultures and that the addition of lower concentrations of GA3 in the medium may provide an improved balance among the endogenous GA in the cultured cells.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Book
Methods of Enzymatic Analysis
TL;DR: Methods of enzymatic analysis, Methods of enzymes analysis, the authors, Methods of enzyme analysis, enzymatics, methods of enzymes, and methods of analysis, method of enzymes.
Journal ArticleDOI
A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples
TL;DR: The original Lowry method of protein determination has been modified by the addition of sodium dodecyl sulfate in the alkali reagent and an increase in the amount of copper tartrate reagent to be used with membrane and lipoprotein preparations without prior solubilization or lipid extraction.