Book ChapterDOI
Quantitation of protein.
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TLDR
This chapter discusses various methods of estimating protein concentration as defined by the difference in energy between the orbital of the unexcited electron and a higher energy orbital.Abstract:
Publisher Summary This chapter discusses various methods of estimating protein concentration. Absorption spectroscopy involves the absorption of a photon by an electron. Only those photons with a certain energy level can be absorbed as defined by the difference in energy between the orbital of the unexcited electron and a higher energy orbital. The peptide bond absorbs photons below 210 nm. Because of the large number of peptide bonds in a protein, this is a highly sensitive area of the protein spectrum. Although protein conformation and some absorption by tryptophan and tyrosine residues occurs in this region, less variability between proteins is observed than at 280 nm. There is a need to avoid storing buffers in plastic containers because some plastics leach plasticizers, which absorb at ultraviolet (UV) wavelengths. Detergents can also be troublesome because many absorb UV light. If the buffer or protein solution is cold, the outside of the cuvette may need to be wiped between each reading with a lint-free wiper and the readings should be made quickly after placing the cold solution into the cuvette, because atmospheric moisture may condense on the outside of the cuvette producing an erroneously high reading.read more
Citations
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Journal ArticleDOI
Head-to-Tail Cyclization after Interaction with Trypsin: A Scorpion Venom Peptide that Resembles Plant Cyclotides.
Caroline Barbosa Farias Mourão,Guilherme D. Brand,João Paulo Campos Fernandes,Maura V. Prates,Carlos Bloch,João Alexandre Ribeiro Gonçalves Barbosa,Sonia Maria de Freitas,Rita Restano-Cassulini,Lourival D. Possani,Elisabeth F. Schwartz +9 more
TL;DR: The X-ray structure of the ToPI1:trypsin complex, in association with the mass spectrometry data, indicate a sequential set of events: the complex formation with the inhibitor Lys32 in the trypsin S1 pocket, the inhibitor C-terminal residue Ser33 cleavage, and the cyclization of To PI1 via a peptide bond between residues Ile1 and Lys32.
Journal ArticleDOI
Bothrops jararaca fibrinogen and its resistance to hydrolysis evoked by snake venoms.
Carolina Okamoto Vieira,Aparecida S. Tanaka,Ida S. Sano-Martins,Karen Batista de Morais,Marcelo L. Santoro,Anita Mitico Tanaka-Azevedo +5 more
TL;DR: Despite the similarities between B.jararaca and mammalian fibrinogens, the former shows distinctive features, which protect B. jararaca snakes from accidental envenomation.
Journal ArticleDOI
Whitefish ( Coregonus lavaretus ) Response to Varying Potassium and Sodium Concentrations: A Model of Mining Water Toxic Response
Ekaterina Borvinskaya,Ekaterina Borvinskaya,Irina Sukhovskaya,Olga Vasil’eva,M. A. Nazarova,L. P. Smirnov,S.A. Svetov,N.V. Krutskikh +7 more
TL;DR: In this paper, the effects of potassium and the potassium: sodium (K+: Na+) ratio in freshwater on growth performance and oxidative stress in a typical northern species of whitefish, Coregonus lavaretus, were investigated.
Journal ArticleDOI
Regulation of the development of flight muscles in long-winged adults of the flightless bug Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae)
Radomír Socha,Jan Šula +1 more
TL;DR: The results indicate that imaginal growth and histolysis of indirect flight muscles in macropterous individuals of P. apterus are largely juvenile hormone-independent processes that are programmed to occur spontaneously, but can be affected by various internal and external factors.
Journal ArticleDOI
Self-assembled polyelectrolyte complexes of chitosan and fucoidan for sustained growth factor release from PRP enhance proliferation and collagen deposition in diabetic mice
TL;DR: A simple but effective polymer system to increase the shelf-life of PRP is demonstrated by developing a polyelectrolyte complex with dropwise addition of chitosan solution containing PRP by simple mixing at room temperature.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
Book
Methods of Enzymatic Analysis
TL;DR: Methods of enzymatic analysis, Methods of enzymes analysis, the authors, Methods of enzyme analysis, enzymatics, methods of enzymes, and methods of analysis, method of enzymes.
Journal ArticleDOI
A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples
TL;DR: The original Lowry method of protein determination has been modified by the addition of sodium dodecyl sulfate in the alkali reagent and an increase in the amount of copper tartrate reagent to be used with membrane and lipoprotein preparations without prior solubilization or lipid extraction.