Quantitative Abnormalities of Fetal DNA in Maternal Serum in Preeclampsia
Y.M. Dennis Lo,Tse N. Leung,Mark S.C. Tein,Ian L. Sargent,Jun Zhang,Tze K. Lau,Christopher J. Haines,Christopher W.G. Redman +7 more
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TLDR
The results suggest that preeclampsia is associated with disturbances in the liberation and/or clearance mechanisms of circulating DNA and raise the possibility that measurement of circulatingDNA may prove useful as a marker for the diagnosis and/ or monitoring of preeClampsia.Abstract:
Background: There is much recent interest in the biologic and diagnostic implication of cell-free non-host DNA in the plasma and serum of human subjects. To determine if quantitative abnormalities of circulating non-host DNA may be associated with certain pathologic processes, we used circulating fetal DNA in preeclampsia as a model system. Methods: We studied 20 preeclamptic women and 20 control subjects of comparable gestational age (means, 32 and 33 weeks, respectively). Male fetal DNA in maternal serum was measured using real-time quantitative PCR for the SRY gene on the Y chromosome. Results: The imprecision (CV) of the assay was 2.7%. The median circulating fetal DNA was increased fivefold in 20 preeclamptic women compared with 20 control pregnant women (381 vs 76 genome-equivalents/mL, P <0.001). Conclusions: These observations suggest that preeclampsia is associated with disturbances in the liberation and/or clearance mechanisms of circulating DNA. These results also raise the possibility that measurement of circulating DNA may prove useful as a marker for the diagnosis and/or monitoring of preeclampsia.read more
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The preterm parturition syndrome
Roberto Romero,Jimmy Espinoza,Juan Pedro Kusanovic,Francesca Gotsch,Sonia S. Hassan,Offer Erez,Tinnakorn Chaiworapongsa,Moshe Mazor +7 more
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Pre-eclampsia, the placenta and the maternal systemic inflammatory response--a review.
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Microfluidics digital PCR reveals a higher than expected fraction of fetal DNA in maternal plasma.
TL;DR: Microfluidics digital PCR represents an improvement over previous methods for quantifying fetal DNA in maternal plasma, enabling diagnostic and research applications requiring precise quantification, and may also impact other diagnostic applications of plasma nucleic acids.
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Placental Debris, Oxidative Stress and Pre-eclampsia
TL;DR: The problem with the placenta is generally considered to be an inadequate uteroplacental circulation leading to placental hypoxia, oxidative stress and, in the most severe cases, infarction.
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Circulating cytokines, chemokines and adhesion molecules in normal pregnancy and preeclampsia determined by multiplex suspension array.
TL;DR: According to the findings, preeclampsia was associated with an overall pro-inflammatory systemic environment and Elevated amounts of pro- inflammatory cytokines, chemokines and adhesion molecules in the maternal circulation might play a central role in the excessive systemic inflammatory response, as well as in the generalized endothelial dysfunction characteristics of the maternal syndrome of preeclamping.
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Presence of fetal DNA in maternal plasma and serum
Y.M. Dennis Lo,Noemi Corbetta,Paul Chamberlain,Vik Rai,Ian L. Sargent,Christopher W.G. Redman,James S. Wainscoat +6 more
TL;DR: The finding of circulating fetal DNA in maternal plasma may have implications for non-invasive prenatal diagnosis, and for improving the understanding of the fetomaternal relationship.
Journal ArticleDOI
Quantitative Analysis of Fetal DNA in Maternal Plasma and Serum: Implications for Noninvasive Prenatal Diagnosis
Y.M. Dennis Lo,Mark S.C. Tein,Tze K. Lau,Christopher J. Haines,Tse N. Leung,Priscilla M.K. Poon,James S. Wainscoat,Philip J. Johnson,Allan M. Z. Chang,N. Magnus Hjelm +9 more
TL;DR: In this paper, a real-time quantitative PCR assay was developed to measure the concentration of fetal DNA in maternal plasma and serum, and the results showed that fetal DNA is present in high concentrations in maternal placenta, reaching a mean of 25.4 genome equivalents/ml (range 3.3-69.4) in early pregnancy and 292.2 genome equivalents /ml(range 76.9-769) in late pregnancy.