Quantitative Calcium Measurements in Subcellular Compartments of Plasmodium falciparum-infected Erythrocytes
Petra Rohrbach,Oliver Friedrich,Oliver Friedrich,Joachim Hentschel,Helmut Plattner,Rainer H. A. Fink,Michael Lanzer +6 more
TLDR
Whether the food vacuole is an intracellular Ca2+ store, which in turn may affect other physiological functions in which this organelle partakes, is investigated using x-ray microanalysis and quantitative confocal live cell Ca 2+ imaging.About:
This article is published in Journal of Biological Chemistry.The article was published on 2005-07-29 and is currently open access. It has received 94 citations till now. The article focuses on the topics: Food vacuole & Ion homeostasis.read more
Citations
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疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
Journal ArticleDOI
Design strategies for water-soluble small molecular chromogenic and fluorogenic probes.
Journal ArticleDOI
Fluorescent indicators for intracellular pH.
Junyan Han,Kevin Burgess +1 more
TL;DR: Various Indicators for Near-Neutral pH Values and Design of pH-Sensitive Cyanine Dyes and Miscellaneous Small Molecule pHi Indicators are presented.
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Genetic linkage of pfmdr1 with food vacuolar solute import in Plasmodium falciparum
Petra Rohrbach,Cecilia P. Sanchez,Karen Hayton,Oliver Friedrich,Jigar Patel,Amar Bir Singh Sidhu,Michael T. Ferdig,David A. Fidock,Michael Lanzer +8 more
TL;DR: A surrogate assay for Pgh‐1 function is described based on the subcellular distribution of Fluo‐4 acetoxymethylester and its free fluorochrome, and it is proposed that Pgh-1 imports solutes, including certain antimalarial drugs, into the parasite's food vacuole.
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The calcium signaling toolkit of the Apicomplexan parasites Toxoplasma gondii and Plasmodium spp.
TL;DR: Apicomplexan genomes reveal a rich repertoire of genes involved in calcium signaling, although many of the genes responsible for observed physiological changes remain unknown as discussed by the authors, and the molecular components involved in Ca(2+) entry in both Toxoplasma and Plasmodium, have not been identified.
References
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Journal ArticleDOI
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
TL;DR: A new family of highly fluorescent indicators has been synthesized for biochemical studies of the physiological role of cytosolic free Ca2+ using an 8-coordinate tetracarboxylate chelating site with stilbene chromophores that offer up to 30-fold brighter fluorescence.
疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
Journal ArticleDOI
Human malaria parasites in continuous culture
William Trager,James B. Jensen +1 more
TL;DR: Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38 degrees C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen.
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Calcium signalling: dynamics, homeostasis and remodelling
TL;DR: The Ca2+-signalling toolkit is used to assemble signalling systems with very different spatial and temporal dynamics and has a direct role in controlling the expression patterns of its signalling systems that are constantly being remodelled in both health and disease.
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Artemisinins target the SERCA of Plasmodium falciparum
Ursula Eckstein-Ludwig,Richard Webb,I. D. A. van Goethem,J M East,Anthony G. Lee,Masatsugu Kimura,Paul M. O'Neill,Patrick G. Bray,Stephen A. Ward,Sanjeev Krishna +9 more
TL;DR: It is shown that artemisinins, but not quinine or chloroquine, inhibit the SERCA orthologue (PfATP6) of Plasmodium falciparum in Xenopus oocytes with similar potency to thapsigargin (another sesquiterpene lactone and highly specific SERCA inhibitor).