Journal ArticleDOI
Quantitative detection of Lactarius deliciosus extraradical soil mycelium by real-time PCR and its application in the study of fungal persistence and interspecific competition.
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TLDR
It is concluded that real-time PCR is a powerful technique for extraradical mycelium quantification in studies aimed at evaluating the persistence of introduced strains of L. deliciosus in field plantations.About:
This article is published in Journal of Biotechnology.The article was published on 2007-01-30. It has received 62 citations till now. The article focuses on the topics: Lactarius deliciosus & Rhizopogon roseolus.read more
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Root tip competition among ectomycorrhizal fungi: Are priority effects a rule or an exception?
TL;DR: Results confirm that priority effects do play a major role in dynamics of EM root tip colonization, at least in the early colonization of seedlings, and that the proportion of the root system occupied by a species appears to be a key factor determining competitive success.
Journal ArticleDOI
Evaluation of methods to estimate production, biomass and turnover of ectomycorrhizal mycelium in forests soils : A review
Håkan Wallander,Alf Ekblad,Douglas L. Godbold,David W. Johnson,Adam Bahr,Petr Baldrian,Robert G. Björk,Barbara Kieliszewska-Rokicka,Rasmus Kjøller,Hojka Kraigher,Claude Plassard,Maria Rudawska +11 more
TL;DR: The need to extend the application of current methods to focus on a greater range of habitats and mycorrhizal types enabling incorporation of mycor rhizal fungal biomass and turnover into biogeochemical cycling models is highlighted.
Journal ArticleDOI
Ectomycorrhizal fungi and interspecific competition: species interactions, community structure, coexistence mechanisms, and future research directions
TL;DR: This review examines the progress that has been made in understanding the nature of EMF competition and highlights recent studies documenting links between competitive coexistence and EMF community structure, including checkerboard distributions, lottery models, storage effects, and colonization-competition tradeoffs.
Journal ArticleDOI
Estimation of fungal biomass in forest litter and soil
Petr Baldrian,Tomáš Větrovský,Tomáš Cajthaml,Petra Dobiášová,Mirka Petránková,Jaroslav Šnajdr,Ivana Eichlerová +6 more
TL;DR: The estimates of fungal biomass obtained with different biomarkers are not exactly comparable, and caution should be used when analysing taxon abundance using PCR amplification offungal rDNA.
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Extramatrical mycelia of ectomycorrhizal fungi as moderators of carbon dynamics in forest soil
TL;DR: Responses of grazing soil arthropods to future climate change will have a strong influence on EMM turnover, along with the abilities of ECM fungi to store carbon in below-ground, and this should be seen as a priority area for future research.
References
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Journal ArticleDOI
ITS primers with enhanced specificity for basidiomycetes--application to the identification of mycorrhizae and rusts.
M. Gardes,Thomas D. Bruns +1 more
TL;DR: In this paper, two taxon-selective primers for the internal transcribed spacer (ITS) region in the nuclear ribosomal repeat unit were proposed, which were intended to be specific to fungi and basidiomycetes, respectively.
Journal ArticleDOI
Real time quantitative PCR.
TL;DR: Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays.
Journal ArticleDOI
Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.
TL;DR: It is proposed that the larger signal in the 5' nuclease PCR assay is caused by increased likelihood of cleavage by Taq DNA polymerase when the probe is hybridized to a template strand during PCR.
BookDOI
Working with mycorrhizas in forestry and agriculture.
TL;DR: In this paper, the authors present an ACIAR-sponsored workshop to promote the use of mycorrhizal fungi for eucalypt plantation forestry in China, and present procedures used by scientists who work with mycophyllus and roots in the laboratory, nursery, or natural and managed ecosystems.