Journal ArticleDOI
Rapid genomic evolution of a non-virulent coxsackievirus B3 in selenium-deficient mice results in selection of identical virulent isolates.
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To the best of the knowledge, this is the first report of a specific nutritional deficiency driving changes in a viral genome, permitting an avirulent virus to acquire virulence due to genetic mutation.Abstract:
Previous work from our laboratory demonstrated that selenium deficiency in the mouse allows a normally benign (amyocarditic) cloned and sequenced Coxackievirus to cause significant heart damage. Furthermore, Coxsackievirus recovered from the hearts of selenium-deficient mice inoculated into selenium-adequate mice still induced significant heart damage, suggesting that the amyocarditic Coxsackievirus had mutated to a virulent phenotype. Here we report that sequence analysis revealed six nucleotide changes between the virulent virus recovered from the selenium-deficient host and the avirulent input virus. These nucleotide changes are consistent with known differences in base composition between virulent and avirulent strains of Coxsackievirus. To the best of our knowledge, this is the first report of a specific nutritional deficiency driving changes in a viral genome, permitting an avirulent virus to acquire virulence due to genetic mutation.read more
Citations
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Journal ArticleDOI
Sendai virus intra-host population dynamics and host immunocompetence influence viral virulence during in vivo passage.
José Peña,Haiyin Chen-Harris,Jonathan E. Allen,Mona Hwang,Maher Elsheikh,Shalini Mabery,Helle Bielefeldt-Ohmann,Adam Zemla,Richard A. Bowen,Monica K. Borucki +9 more
TL;DR: The diet of animal model reflected two extremes of diets which exist in the global population, malnutrition and obesity, and revealed the role of viral mutant spectra dynamics and host immunocompetence in the development of pathogenicity during in vivo passage.
Journal Article
Cervical carcinogenesis: The role of co-factors and generation of reactive oxygen species. [Carcinogénesis cervical: co-factores y antioxidantes.]
TL;DR: Results from the numerous epidemiological studies conducted to date indicate that cervical dysplasia and cancer may be reduced if the oxidant antioxidant ratio is shifted to more of and antioxidant profile.
Patent
Selenoproteins, coding sequences and methods
TL;DR: In this article, a method for the identification of nucleotide sequences which encode selenoproteins was proposed, and frameshift-dependent open reading frames and SECIS elements were identified as associated with selenope protein coding sequences.
Selenium nanoparticles role in organ systems functionality and disorder
TL;DR: The intent of this review is to investigate current knowledge of SeNPs interaction with major body organ systems functionality, and Investigated pharmacokinetic parameters ofSeNPs was also reviewed.
Book ChapterDOI
Error Frequencies of Picornavirus RNA Polymerases: Evolutionary Implications for Virus Populations
TL;DR: This chapter examines key features of the three main stages in virus evolution with examples drawn from several virus groups, and reviews specific results with picornaviruses, as well as implications of high mutation rates and quasispecies dynamics for this important and diverse group of pathogens.
References
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Journal ArticleDOI
Oxidants, antioxidants, and the degenerative diseases of aging
TL;DR: It is argued that this damage to DNA, protein, and lipid is a major contributor to aging and to degenerative diseases of aging such as cancer, cardiovascular disease, immune-system decline, brain dysfunction, and cataracts.
Journal ArticleDOI
Rapid evolution of RNA genomes
John J. Holland,Katherine R. Spindler,Katherine R. Spindler,Frank M. Horodyski,Elizabeth A. Grabau,Stuart T. Nichol,Scott VandePol +6 more
TL;DR: RNA viruses show high mutation frequencies partly because of a lack of the proofreading enzymes that assure fidelity of DNA replication, and high rates of replication reflected in rates of RNA genome evolution which can be more than a millionfold greater than the rates of the DNA chromosome evolution of their hosts.
Journal ArticleDOI
Spontaneous point mutations that occur more often when advantageous than when neutral.
TL;DR: It is shown that point mutations in the trp operon reverted to trp+ more frequently under conditions of prolonged tryptophan deprivation when the reversions were advantageous, than in the presence of tryPTophan when the reversal were neutral, and a heuristic model for the molecular basis of such mutations is proposed.
Journal ArticleDOI
Complete nucleotide sequence of infectious Coxsackievirus B3 cDNA: two initial 5' uridine residues are regained during plus-strand RNA synthesis.
TL;DR: It is reported that cDNA-generated CVB3, as well asCVB3 generated by in vitro-synthesized RNA transcripts, regains the authentic initial 5' uridine residues during replication in transfected cells, indicating that the picornaviral primer molecule VPg-pUpU may be uridylylated in a template-independent fashion.