Journal ArticleDOI
Rapid genomic evolution of a non-virulent coxsackievirus B3 in selenium-deficient mice results in selection of identical virulent isolates.
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TLDR
To the best of the knowledge, this is the first report of a specific nutritional deficiency driving changes in a viral genome, permitting an avirulent virus to acquire virulence due to genetic mutation.Abstract:
Previous work from our laboratory demonstrated that selenium deficiency in the mouse allows a normally benign (amyocarditic) cloned and sequenced Coxackievirus to cause significant heart damage. Furthermore, Coxsackievirus recovered from the hearts of selenium-deficient mice inoculated into selenium-adequate mice still induced significant heart damage, suggesting that the amyocarditic Coxsackievirus had mutated to a virulent phenotype. Here we report that sequence analysis revealed six nucleotide changes between the virulent virus recovered from the selenium-deficient host and the avirulent input virus. These nucleotide changes are consistent with known differences in base composition between virulent and avirulent strains of Coxsackievirus. To the best of our knowledge, this is the first report of a specific nutritional deficiency driving changes in a viral genome, permitting an avirulent virus to acquire virulence due to genetic mutation.read more
Citations
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Selenium from High Selenium Broccoli Protects Rats from Colon Cancer
TL;DR: Adequate dietary Se supplied as high Se broccoli did not accumulate in tissues or increase glutathione peroxidase activity as well as other forms and amounts of Se.
Journal ArticleDOI
Dietary Selenium in Adjuvant Therapy of Viral and Bacterial Infections
TL;DR: A recent review as mentioned in this paper provides an up-to-date overview on selenium in infectious diseases caused by viruses (e.g., HIV, IAV, hepatitis C virus, poliovirus, West Nile virus) and bacteria (i.e., M. tuberculosis, Helicobacter pylori).
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Glutathione peroxidase protects mice from viral-induced myocarditis
TL;DR: Results demonstrate that GPX‐1 provides protection against viral‐induced damage in vivo due to mutations in the viral genome of a benign virus.
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High-selenium wheat: biofortification for better health.
TL;DR: Before recommending large-scale fortification of the food supply with Se, it will be necessary to await the results of current intervention studies with Se on cancer, HIV and AIDS, and asthma.
Journal ArticleDOI
Understanding the importance of selenium and selenoproteins in muscle function
TL;DR: The goal of this review is to establish the connection between clinical observations and the most recent advances obtained in selenium biology, and to understand the muscle disorders associated with selenum deficiency or selenoprotein N dysfunction.
References
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Journal ArticleDOI
Oxidants, antioxidants, and the degenerative diseases of aging
TL;DR: It is argued that this damage to DNA, protein, and lipid is a major contributor to aging and to degenerative diseases of aging such as cancer, cardiovascular disease, immune-system decline, brain dysfunction, and cataracts.
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Rapid evolution of RNA genomes
John J. Holland,Katherine R. Spindler,Katherine R. Spindler,Frank M. Horodyski,Elizabeth A. Grabau,Stuart T. Nichol,Scott VandePol +6 more
TL;DR: RNA viruses show high mutation frequencies partly because of a lack of the proofreading enzymes that assure fidelity of DNA replication, and high rates of replication reflected in rates of RNA genome evolution which can be more than a millionfold greater than the rates of the DNA chromosome evolution of their hosts.
Journal ArticleDOI
Spontaneous point mutations that occur more often when advantageous than when neutral.
TL;DR: It is shown that point mutations in the trp operon reverted to trp+ more frequently under conditions of prolonged tryptophan deprivation when the reversions were advantageous, than in the presence of tryPTophan when the reversal were neutral, and a heuristic model for the molecular basis of such mutations is proposed.
Journal ArticleDOI
Complete nucleotide sequence of infectious Coxsackievirus B3 cDNA: two initial 5' uridine residues are regained during plus-strand RNA synthesis.
TL;DR: It is reported that cDNA-generated CVB3, as well asCVB3 generated by in vitro-synthesized RNA transcripts, regains the authentic initial 5' uridine residues during replication in transfected cells, indicating that the picornaviral primer molecule VPg-pUpU may be uridylylated in a template-independent fashion.