Journal ArticleDOI
Reconstitution of membranes with individual paramyxovirus glycoproteins and phospholipid in cholate solution.
TLDR
The results have confirmed the role of the F protein in membrane fusion and have shown that the requirements for F protein activity are the previously demonstrated proteolytic processing of F, the insertion of theF protein into lipid, and the presence of an attachment mechanism.About:
This article is published in Virology.The article was published on 1979-06-01. It has received 167 citations till now. The article focuses on the topics: HN Protein & Phosphatidylcholine.read more
Citations
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Journal ArticleDOI
Endoproteolytic cleavage of gp160 is required for the activation of human immunodeficiency virus
Joseph M. McCune,Linda Rabin,Mark B. Feinberg,Miriam Lieberman,Jon C. Kosek,Gregory R. Reyes,Irving L. Weissman +6 more
TL;DR: In this study, the tryptic-like endoproteolytic cleavage site was removed by site-directed mutagenesis and replaced with a chymotryptic-like site and the resultant mutant, RIP7/mut10, was found to be indistinguishable from wild-type HIV when analyzed at the level of proviral replication, RNA processing, protein expression, and viral assembly.
Journal ArticleDOI
Membrane fusion proteins of enveloped animal viruses.
TL;DR: Although a great deal is known about the properties and consequences of individual forms of membrane fusion in cellular systems, and about fusion in artificial lipid membranes, the molecular basis for the reactions remain largely unclear.
Journal ArticleDOI
The role of viral glycoproteins in adsorption, penetration, and pathogenicity of viruses.
TL;DR: The F Glycoprotein 43 Protease activation mutants of a paramyxovirus 45 The activating protease 45 Proteolytic cleavage and viral virulence ..... 46 structure of the F protein 47 Specific inhibition of virus-induced membrane fusion and virus penetration by oligopeptides 48 Importance of antibodies to F glycoprotein in prevention of spread of paramyxvirus infection 49
Journal ArticleDOI
Importance of antibodies to the fusion glycoprotein of paramyxoviruses in the prevention of spread of infection.
TL;DR: Results indicate that effective immunological prevention of the spread of paramyxovirus infection requires the presence of antibodies that inactivate the F glycoprotein, which has obvious implications for the design of effective paramxovirus vaccines.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids.
Journal ArticleDOI
Quantitative analysis of phospholipids by thin-layer chromatography and phosphorus analysis of spots.
TL;DR: The method reported here has the advantage of improved separations by two-dimensionM TLC, direct aspiration of spots by suction, and phosphorus analysis without pr ior elution.
Related Papers (5)
Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus.
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Trypsin Action on the Growth of Sendai Virus in Tissue Culture Cells III. Structural Difference of Sendai Viruses Grown in Eggs and Tissue Culture Cells
Morio Homma,Masanobu Ohuchi +1 more