Recovery of herbicide-resistant Azuki bean [ Vigna angularis (Wild.), Ohwi & Ohashi] plants via Agrobacterium -mediated transformation
TLDR
The presence of transgenes in transformed azuki bean plants was confirmed by polymerase chain reaction (PCR) and southern blot analysis and demonstrates the feasibility of introducing potentially useful agronomic traits into azukibean through genetic engineering.Abstract:
Transgenic azuki bean [ Vigna angularis (Willd.) Ohwi & Ohashi] plants expressing the hygromycin phosphotransferase ( hpt ), green fluorescent protein ( sgfp ) and phosphinothricin acetyltransferase ( bar ) genes were obtained by Agrobacterium- tumefacients - mediated transformation. A total of 210 epicotyl explants were inoculated with A. tumefaciens strain EHA105, harboring the binary plasmid pZHBG on MS co-cultivation medium supplemented with 100 mM acetosyringone and 10 mg/l of BA. Following selection on MS medium with 15 mg/l of hygromycin, the regenerated adventitious shoots that formed on the induced calli were further screened for sgfp expression before transferred to rooting medium. 31 transgenic plants were obtained with transformation frequency of 14%. The presence of transgenes in transformed azuki bean plants was confirmed by polymerase chain reaction (PCR) and southern blot analysis. Transcription of the bar and hpt genes was assessed by reverse transcription polymerase chain reaction (RT-PCR) analysis. sgfp- positive transgenic plants exhibited functional expression of the bar gene as determined by assaying for resistance to bialaphos applied directly to leaves. This result demonstrates the feasibility of introducing potentially useful agronomic traits into azuki bean through genetic engineering. Key Words: Agrobacterium tumefaciens, bar gene, bialaphos, transgenic, Vigna angulazris . African Journal of Biotechnology Vol.4(1) 2005: 61-67read more
Citations
More filters
Journal ArticleDOI
Advances in development of transgenic pulse crops.
TL;DR: With optimization of various factors which influence genetic transformation of pulse crops, it will be possible to develop transgenic plants in this important group of crop species with more precision and reproducibility, according to the reason for lack of commercialization of transgenic pulse crops.
Journal ArticleDOI
Agrobacterium tumefaciens mediated transfer of Phaseolus vulgaris α-amylase inhibitor-1 gene into mungbean Vigna radiata (L.) Wilczek using bar as selectable marker
TL;DR: Morphologically normal and fertile transgenic plants of mungbean with two transgenes, bar and α-amylase inhibitor, have been developed for the first time and inheritance of both thetransgenes in most of the T1 lines was revealed.
Journal ArticleDOI
Organogenesis of Phaseolus angularis L.: high efficiency of adventitious shoot regeneration from etiolated seedlings in the presence of N6-benzylaminopurine and thidiazuron
TL;DR: A step-wise procedure for the regeneration of fertile plants by organogenesis from cultures of the economically important Phaseolus angularis L., cultivars: KS-6, KS-7 and KS-8 using etiolated seedlings was established, finding the efficient shoot bud induction capability to be cultivar dependent.
Journal ArticleDOI
Genomics and molecular breeding in lesser explored pulse crops: current trends and future opportunities.
TL;DR: This article examines the prospects of genomics assisted integrated breeding to enhance and stabilize crop yields and outlines the recent progress made in genomics of these lesser explored pulse crops.
Book ChapterDOI
Adzuki Bean (Vigna angularis (Willd.) Ohwi & Ohashi) Breeding
Lixia Wang,Jie Wang,Xuzhen Cheng +2 more
TL;DR: DNA marker analysis suggests that there are obvious genetic distinctions between different forms, but the diversity among cultivated germplasm is quite low, indicating that the wild forms could be an important genetic resource for breeding.
References
More filters
Journal ArticleDOI
Transgenic Pea Seeds Expressing the α-Amylase Inhibitor of the Common Bean are Resistant to Bruchid Beetles
Richard E. Shade,Hartmut E. Schroeder,José Javier Pueyo,Linda Tabe,Larry L. Murdock,Thomas J. V. Higgins,Maarten J. Chrispeels +6 more
TL;DR: The levels of αAI protein in the pea seeds were as high as in bean seeds and the peas were resistant to the cowpea and Azuki bean weevils, suggesting this gene is a candidate for a genetic engineering approach that would make other legumes resistant to bruchid infestations.
Journal ArticleDOI
Transformation and Regeneration of Two Cultivars of Pea (Pisum sativum L.)
Hartmut E. Schroeder,Andrea H. Schotz,Terese Wardley-Richardson,Donald Spencer,Thomas J. V. Higgins +4 more
TL;DR: A reproducible transformation system was developed for pea using as explants sections from the embryonic axis of immature seeds, and transformed plants were resistant to the herbicide Basta when sprayed at rates used in field practice.
Journal ArticleDOI
Transformation of Soybean (Glycine max) by Infecting Germinating Seeds with Agrobacterium tumefaciens.
TL;DR: The transfer of genetic material into soybean tissue was accomplished by using an avirulent strain of Agrobacterium tumefaciens which contained the binary vector pGA482, and the presence of the Nos-NPT II gene in DNAs isolated from both R(0) and R(1) plant was demonstrated by using genomic blot hybridization and polymerase chain reaction methods.
Journal ArticleDOI
Production of transgenic pea (Pisum sativum L.) plants by Agrobacterium tumefaciens - mediated gene transfer.
TL;DR: A transformation system that allows regeneration of transgenic pea plants from calli selected for antibiotic resistance was developed and the transformation of the calli and regenerated plants was confirmed by DNA analysis.
Journal ArticleDOI
Genetic transformation in the grain legume Cicer arietinum L. (chickpea).
TL;DR: In the grain legume Cicer arietinum L. (chickpea), the seed-derived embryo axes deprived of the apical meristem were able to regenerate adventitious shoots on Murashige and Skoog medium supplemented with kinetin, suitable for Agrobacterium-mediated gene transfer by the co-cultivation technique.