Journal ArticleDOI
Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2
Akila Mayeda,Adrian R. Krainer +1 more
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TLDR
When messenger RNA precursors (pre-mRNAs) containing alternative 5' splice sites are spliced in vitro, the relative concentrations of the heterogeneous ribonucleoprotein (hnRNP) A1 and the essential splicing factor SF2 precisely determine which 5'splice site is selected.About:
This article is published in Cell.The article was published on 1992-01-24. It has received 692 citations till now. The article focuses on the topics: Exonic splicing enhancer & Alternative splicing.read more
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Journal ArticleDOI
Mechanisms of Alternative Pre-Messenger RNA Splicing
TL;DR: This review describes what is currently known of the molecular mechanisms that control changes in splice site choice and starts with the best-characterized systems from the Drosophila sex determination pathway, and then describes the regulators of other systems about whose mechanisms there is some data.
Journal ArticleDOI
Conserved structures and diversity of functions of RNA-binding proteins.
TL;DR: The major RNA-binding motifs are described and examples of how they may function are given.
Journal ArticleDOI
Messenger-RNA-binding proteins and the messages they carry.
TL;DR: From sites of transcription in the nucleus to the outreaches of the cytoplasm, messenger RNAs are associated with RNA-binding proteins that communicate crucial information to the translation machinery for the surveillance of nonsense mutations and for mRNA localization and translation.
Journal ArticleDOI
Understanding alternative splicing: towards a cellular code.
TL;DR: Traditional gene-by-gene investigations of alternative splicing mechanisms are now being complemented by global approaches that promise to reveal details of the nature and operation of cellular codes that are constituted by combinations of regulatory elements in pre-mRNA substrates and by cellular complements of splicing regulators, which together determine regulated splicing pathways.
Journal ArticleDOI
The SR protein family of splicing factors: master regulators of gene expression.
TL;DR: It is demonstrated how SR proteins are emerging as one of the master regulators of gene expression as well as pivotal regulators of mRNA metabolism, and if these functions are disrupted, developmental defects or disease may result.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Book ChapterDOI
Use of T7 RNA polymerase to direct expression of cloned genes.
Journal ArticleDOI
Arginine-mediated RNA recognition: the arginine fork.
TL;DR: Model building suggests that the arginine eta nitrogens and the epsilon nitrogen can form specific networks of hydrogen bonds with adjacent pairs of phosphates and that these arrangements are likely to occur near RNA loops and bulges and not within double-stranded A-form RNA.