Journal ArticleDOI
Regulation of cytokinesis by the Elm1 protein kinase in Saccharomyces cerevisiae
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TLDR
Genetic and biochemical evidence suggest that Elm1 and the three other septin-localised protein kinases work in parallel pathways to regulate septin behaviour and cytokinesis in budding yeast is regulated by Elm1.Abstract:
A Saccharomyces cerevisiae mutant unable to grow in a cdc28-1N background was isolated and shown to be affected in the ELM1 gene. Elm1 is a protein kinase, thought to be a negative regulator of pseudo-hyphal growth. We show that Cdc11, one of the septins, is delocalised in the mutant, indicating that septin localisation is partly controlled by Elm1. Moreover, we show that cytokinesis is delayed in an elm1delta mutant. Elm1 levels peak at the end of the cell cycle and Elm1 is localised at the bud neck in a septin-dependent fashion from bud emergence until the completion of anaphase, at about the time of cell division. Genetic and biochemical evidence suggest that Elm1 and the three other septin-localised protein kinases, Hsl1, Gin4 and Kcc4, work in parallel pathways to regulate septin behaviour and cytokinesis. In addition, the elm1delta;) morphological defects can be suppressed by deletion of the SWE1 gene, but not the cytokinesis defect nor the septin mislocalisation. Our results indicate that cytokinesis in budding yeast is regulated by Elm1.read more
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Activation of yeast Snf1 and mammalian AMP-activated protein kinase by upstream kinases
TL;DR: Three yeast kinases are identified that activate Snf1 kinase in vivo and it is shown that the closely related mammalian LKB1 kinases, which is associated with Peutz–Jeghers cancer-susceptibility syndrome, phosphorylates and activates AMPK in vitro.
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Mammalian TAK1 activates Snf1 protein kinase in yeast and phosphorylates AMP-activated protein kinase in vitro.
TL;DR: Genetic and biochemical evidence that TAK1 is a functional member of the Snf1/AMPK kinase family and support Taker1 as a candidate for an authentic AMPK kinase in mammalian cells are presented.
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SNF1/AMPK pathways in yeast.
TL;DR: The current understanding of SNF1 protein kinase pathways in Saccharomyces cerevisiae and other yeasts is reviewed.
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Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and Beyond
Hay-Oak Park,Erfei Bi +1 more
TL;DR: This review discusses the key signaling pathways that regulate cell polarization during the mitotic cell cycle and during mating and how these GTPases are regulated.
Journal ArticleDOI
The septin cortex at the yeast mother-bud neck.
TL;DR: Recent findings suggest that this domain serves as a diffusion barrier and also as a local cell-shape sensor and what is known about the organization of the septin cortex and its regulation during the cell cycle is reviewed.
References
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Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
Robert S. Sikorski,Philip Hieter +1 more
TL;DR: A series of yeast shuttle vectors and host strains has been created to allow more efficient manipulation of DNA in Saccharomyces cerevisiae to perform most standard DNA manipulations in the same plasmid that is introduced into yeast.
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A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
Journal ArticleDOI
Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
Mark S. Longtine,Amos Mckenzie,Douglas J. Demarini,Nirav Shah,Achim Wach,Arndt Brachat,Peter Philippsen,John R. Pringle +7 more
TL;DR: A new set of plasmids that serve as templates for the PCR synthesis of fragments that allow a variety of gene modifications that should further facilitate the rapid analysis of gene function in S. cerevisiae.
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Comprehensive Identification of Cell Cycle–regulated Genes of the Yeast Saccharomyces cerevisiae by Microarray Hybridization
Paul T. Spellman,Gavin Sherlock,Gavin Sherlock,Michael Q. Zhang,Vishwanath R. Iyer,Kirk R. Anders,Michael B. Eisen,Patrick O. Brown,Patrick O. Brown,David Botstein,Bruce Futcher +10 more
TL;DR: A comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle is created, and it is found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins.