Regulation of luminescence by cyclic AMP in cya-like and crp-like mutants of Vibrio fischeri.
TLDR
Mutants of Vibrio fischeri MJ-1 apparently deficient in adenylate cyclase (cya-like) or cyclic AMP receptor protein (crp- like) were isolated and characterized and addition of cyclicAMP restored wild-type levels of luminescence and luciferase in the cya- like mutant but not in the crp-like mutant.Abstract:
Mutants of Vibrio fischeri MJ-1 (wild type) apparently deficient in adenylate cyclase (cya-like) or cyclic AMP receptor protein (crp-like) were isolated and characterized. Compared with MJ-1, the mutants produced low levels of luminescence and luciferase. Addition of cyclic AMP restored wild-type levels of luminescence and luciferase in the cya-like mutant but not in the crp-like mutant. The results are consistent with the hypothesis that in V. fischeri cyclic AMP and cyclic AMP receptor protein are required for induction of the luminescence system.read more
Citations
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Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators.
TL;DR: How the marine luminescent bacterium V. fischeri uses the LuxR and LuxI proteins for intercellular communication is reviewed and a newly discovered family of LuxRand LuxI homologs in diverse bacterial species is described.
Journal ArticleDOI
Molecular biology of bacterial bioluminescence.
TL;DR: The ability to express the lux genes in a variety of prokaryotic and eukaryotic organisms and the ease and sensitivity of the luminescence assay demonstrate the considerable potential of the widespread application of the Lux genes as reporters of gene expression and metabolic function.
Journal ArticleDOI
Evidence that halogenated furanones from Delisea pulchra inhibit acylated homoserine lactone (AHL)-mediated gene expression by displacing the AHL signal from its receptor protein.
Mike Manefield,Rocky de Nys,Kumar Naresh,Read Roger,Michael Givskov,Steinberg Peter,Staffan Kjelleberg +6 more
TL;DR: The contention that furanones, at the concentrations produced by the alga, can control bacterial colonization of surfaces by specifically interfering with AHL-mediated gene expression at the level of the LuxR protein is supported.
Journal ArticleDOI
Cyclic AMP in prokaryotes.
J. L. Botsford,J G Harman +1 more
TL;DR: This review summarizes recent work focused on elucidating the molecular mechanisms of CRP-cAMP complex-mediated processes in enteric coliforms and suggests a role for cAMP has been suggested in nitrogen fixation.
Journal ArticleDOI
Vibrio fischeri lux Genes Play an Important Role in Colonization and Development of the Host Light Organ
TL;DR: This study is the first to show that the capacity for bioluminescence is critical for normal cell-cell interactions between a bacterium and its animal host and presents the first examples of V. fischeri genes that affect normal host tissue development.
References
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Journal ArticleDOI
A Protein Binding Assay for Adenosine 3′:5′-Cyclic Monophosphate
TL;DR: A simple and sensitive assay for adenosine 3':5'-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase.
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Structural identification of autoinducer of Photobacterium fischeri luciferase.
Anatol Eberhard,Alma L. Burlingame,C. Eberhard,George L. Kenyon,Kenneth H. Nealson,Norman J. Oppenheimer +5 more
TL;DR: In this paper, an autoinducer excreted by Photobacterium fischeri strain MJ-1 was isolated from the cell-free medium by extraction with ethyl acetate, evaporation of solvent, workup with ethanol-water mixtures, and silica gel chromatography.
Isolation and Genetic Analysis of Functions from Vibrio fischeri
TL;DR: Recombinant E. coli that produce light were found in a clone library of hybrid plasmids containing DNA from the marine bacterium Vibrio fischeri, and a model for genetic control of light production is proposed.
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Bacterial bioluminescence: isolation and genetic analysis of functions from Vibrio fischeri.
TL;DR: In this article, a 16-kb insert that encoded enzymatic activities for the light reaction as well as regulatory functions necessary for expression of the luminescence phenotype (Lux) was found in a clone library of hybrid plasmids containing DNA from the marine bacterium Vibrio fischeri.