Reproducibility of random amplified polymorphic DNA (RAPD) analysis among laboratories
G. A. Penner,Aria Bush,Roger P. Wise,Won Kim,Les Domier,K. J. Kasha,André Laroche,Graham J. Scoles,Stephen J. Molnar,George Fedak +9 more
TLDR
Random amplified polymorphic DNA (RAPD) analysis appears to offer a cost- and time-effective alternative to restriction fragment-length polymorphism (RFLP) analysis, but concerns about the ability to compare RAPD results from one laboratory to another have not been addressed effectively.Abstract:
Random amplified polymorphic DNA (RAPD) analysis appears to offer a cost- and time-effective alternative to restriction fragment-length polymorphism (RFLP) analysis. However, concerns about the ability to compare RAPD results from one laboratory to another have not been addressed effectively. DNA fragments that were amplified by five primers and shown to be reproducibly polymorphic between two oat cultivars (within the Ottawa laboratory) were tested in six other laboratories in North America. Four of the six participants amplified very few or no fragments using the Ottawa protocol. These same participants were able to generate a considerable number of amplified fragments by using their own protocols. The reproducibility of results among laboratories was affected by two factors. First, different laboratories amplified different size ranges of DNA fragments, and, consequently, small and large polymorphic fragments were not always reproduced. Second, although reproducible results were obtained with four of the primers, reproducible results were not obtained with the fifth primer, using the same reaction conditions. It is suggested that if the overall temperature profiles (especially the annealing temperature) inside the tubes are identical among the laboratories, then RAPD fragments are likely to be reproducible.read more
Citations
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Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories
C. J. Jones,Keith J. Edwards,S. Castaglione,Mark O. Winfield,Francesco Sala,C.C.M. van de Wiel,G.M.M. Bredemeijer,Ben Vosman,Michaela C. Matthes,A. Daly,Reinhold Brettschneider,P. Bettini,Marcello Buiatti,Elena Maestri,Aliosha Malcevschi,Nelson Marmiroli,R. L. Aert,Guido Volckaert,Julia Rueda,Rosario Linacero,A. M. Vázquez,Angela Karp +21 more
TL;DR: This article describes a network experiment involving several European laboratories, in which the reproducibility of three popular molecular marker techniques was examined: random-amplified fragment length polymorphism (RAPD), amplified fragment length SNP (AFLP) and sequence-tagged microsatellites (SSR).
Journal ArticleDOI
Genome mapping, molecular markers and marker-assisted selection in crop plants
TL;DR: Applications of genome mapping and marker-assisted selection in crop improvement are reviewed and the use of MAS in breeding for disease and pest resistance is considered.
Journal ArticleDOI
Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomy
Paul Janssen,Renata Coopman,Geert Huys,Jean Swings,Marjo Bleeker,Pieter Vos,Marc Zabeau,Karel Kersters +7 more
TL;DR: Comparison of the newly obtained data with results previously obtained by well-established genotypic and chemotaxonomic methods shows the superior discriminative power of AFLP towards the differentiation of highly related bacterial strains that belong to the same species or even biovar (i.e. to characterize strains at the infrasubspecific level).
Book
DNA fingerprinting in plants: principles, methods, and applications
TL;DR: Amin et al. as discussed by the authors used radioisotopes to measure the diversity of microsatellites in the field of plant DNA collection and preservation of plant Tissue in the Field.
Journal ArticleDOI
Genotyping of Campylobacter spp.
TL;DR: The technologies currently available for genotypic subtyping of Campylobacter spp.
References
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DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Journal ArticleDOI
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John Welsh,Michael McClelland +1 more
TL;DR: The generality of the arbitrarily primed PCR method is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa.
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Susan R. McCouch,G. Kochert,Z. H. Yu,Z. Y. Wang,Gurdev S. Khush,W. R. Coffman,Steven D. Tanksley +6 more
TL;DR: It is discovered that rice DNA is less C-methylated than tomato or maize DNA, suggesting the notion that a large fraction of the rice genome (approximately 50%) is single copy.
Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines (plant breeding/polymerase chain reaction/Pto gene/chromosome walking/linkage mapping)
TL;DR: In this article, an approach to isolate DNA sequences that are linked to important plant genes is described, based upon a modification of the polymerase chain reaction in which synthetic primers are used to amplify random sequences from genomic DNA.