Journal ArticleDOI
Resistance of the scrapie agent to inactivation by psoralens.
TLDR
Five psoralens, at concentrations up to 500 times greater than those required to inactivate conventional viruses, did not influence scrapie agent titers in partially purified preparations from murine spleen and hamster brain.Abstract:
–In searching for a nucleic acid within the scrapie agent, we employed psoralens which penetrate the coats of most conventional viruses, form photoadducts with their genomes, and block replication of the viruses. Five psoralens, at concentrations up to 500 times greater than those required to inactivate conventional viruses, did not influence scrapie agent titers in partially purified preparations from murine spleen and hamster brain. 3H-psoralens were used to monitor the formation of photoadducts within nucleic acid standards added to preparations of the scrapie agent. Since no inhibition of psoralen photoadduct formation was observed in these preparations, one of three possibilities seems likely: the scrapie agent is devoid of nucleic acid, the psoralens failed to penetrate the protein coat of the agent, or its nucleic acid is unreactive with psoralens.read more
Citations
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Journal ArticleDOI
Molecular biology of prion diseases
TL;DR: Understanding prion diseases may advance investigations of other neurodegenerative disorders and of the processes by which neurons differentiate, function for decades, and then grow senescent.
Journal ArticleDOI
A cellular gene encodes scrapie PrP 27-30 protein
Bruno Oesch,Bruno Oesch,David Westaway,Monika Wälchli,Monika Wälchli,Michael P. McKinley,Stephen B. H. Kent,Stephen B. H. Kent,Ruedi Aebersold,Ruedi Aebersold,Ronald A. Barry,Paul Tempst,Paul Tempst,David B. Teplow,David B. Teplow,Leroy Hood,Leroy Hood,Stanley B. Prusiner,Stanley B. Prusiner,Charles Weissmann,Charles Weissmann +20 more
TL;DR: A clone encoding PrP 27-30, the major protein in purified preparations of scrapie agent, was selected from a scrapie-infected hamster brain cDNA library by oligonucleotide probes corresponding to the N terminus of the protein.
Journal ArticleDOI
Scrapie prion protein contains a phosphatidylinositol glycolipid
TL;DR: Observations indicate that PrPC is anchored to the cell surface by the glycolipid, which is derived from PrPSc by limited proteolysis at the amino terminus.
Journal ArticleDOI
Scrapie prions aggregate to form amyloid-like birefringent rods
Stanley B. Prusiner,Michael P. McKinley,Karen A. Bowman,David C. Bolton,Paul E. Bendheim,Darlene Groth,George G. Glenner +6 more
TL;DR: The findings raise the possibility that the amyloid plaques observed in transmissible, degenerative neurological diseases might consist of prions, a polymeric form of the scrapie prion.
Journal ArticleDOI
Scrapie and cellular PrP isoforms are encoded by the same chromosomal gene
Konrad Basler,Bruno Oesch,Michael R. Scott,David Westaway,Monika Wälchli,Darlene Groth,Michael P. McKinley,Stanley B. Prusiner,Charles Weissmann +8 more
TL;DR: The primary structure of PrP encoded by the gene of a healthy animal does not differ from that encoded by a cDNA from a scrapie-infected animal, suggesting that the different properties ofPrP from normal and scrapie -infected brains are due to post-translational events.
References
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A cellular gene encodes scrapie PrP 27-30 protein
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