Resistant and susceptible responses in tomato to cyst nematode are differentially regulated by salicylic acid.
TLDR
It is found that PR-1(P4) was a hallmark for the cultivar resistance conferred by Hero A against PCN and that nematode parasitism resulted in the inhibition of the SA signaling pathway in the susceptible cultivars.Abstract:
To understand the machinery underlying a tomato cultivar harboring the Hero A gene against cyst nematode using microarrays, we fi rst analyzed tomato gene expression in response to potato cyst nematode (PCN; Globodera rostochiensis ) during the early incompatible and compatible interactions at 3 and 7 days post-inoculation (dpi). Transcript levels of the phenylalanine ammonia lyase ( PAL ) and Myb related genes were up-regulated at 3 dpi in the incompatible interaction. Transcription of the genes encoding pyruvate decarboxylase ( PDC ) and alcohol dehydrogenase ( ADH ) was also up-regulated at 3 dpi in the incompatible interaction. On the other hand, the four genes ( PAL , Myb , PDC and ADH ) were down-regulated in the compatible interaction at 3 dpi. When the expression levels of several pathogenesis-related (PR) protein genes in tomato roots were compared between the incompatible and compatible interactions, the salicylic acid (SA)-dependent PR genes were found to be induced in the incompatible interaction at 3 dpi. The PR-1 ( P4 ) transcript increased to an exceptionally high level at 3 dpi in the cyst nematode-infected resistant plants compared with the uninoculated controls. The free SA levels were elevated to similar levels in both incompatible and compatible interactions. We then confi rmed that PR-1 ( P4 ) was not signifi cantly induced in the NahG tomato harboring the Hero A gene, compared with the resistant cultivar. We thus found that PR-1 ( P4 ) was a hallmark for the cultivar resistance conferred by Hero A against PCN and that nematode parasitism resulted in the inhibition of the SA signaling pathway in the susceptible cultivars.read more
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TOMATOMA : A Novel Tomato Mutant Database Distributing Micro-Tom Mutant Collections
Takeshi Saito,Tohru Ariizumi,Yoshihiro Okabe,Erika Asamizu,Kyoko Hiwasa-Tanase,Naoya Fukuda,Tsuyoshi Mizoguchi,Yukiko Yamazaki,Koh Aoki,Hiroshi Ezura +9 more
TL;DR: Genetic analysis of backcrosses indicated the successful inheritance of the mutations in BC1F2 populations, confirming the reproducibility in the morphological phenotyping of the M2 plants and developed the in silico database TOMATOMA, a relational system interfacing modules between mutant line names and phenotypic categories.
Journal ArticleDOI
Tomato TILLING Technology: Development of a Reverse Genetics Tool for the Efficient Isolation of Mutants from Micro-Tom Mutant Libraries
Yoshihiro Okabe,Erika Asamizu,Takeshi Saito,Chiaki Matsukura,Tohru Ariizumi,Cécile Bres,Christophe Rothan,Tsuyoshi Mizoguchi,Hiroshi Ezura +8 more
TL;DR: Two allelic mutants of SlETR1 (Sletr 1-1 and Sletr1-2) that resulted in reduced ethylene responses were identified, indicating that the Micro-Tom TILLING platform provides a powerful tool for the rapid detection of mutations in an EMS mutant library.
Journal ArticleDOI
The endophytic strain Fusarium oxysporum Fo47: a good candidate for priming the defense responses in tomato roots.
TL;DR: It is demonstrated that priming of tomato defense responses is one of the mechanisms of action of Fo47, which induces a reduced colonization of the root of the tomato plant preinoculated with Fo47.
Journal ArticleDOI
Expression of tomato salicylic acid (SA)-responsive pathogenesis-related genes in Mi-1-mediated and SA-induced resistance to root-knot nematodes.
TL;DR: The data presented in this study show that the repression of host defence SA signalling is associated with the successful development of R KNs, and that SA exogenously added as a soil drench is able to trigger a SAR-like response to RKNs in tomato.
Book ChapterDOI
The Impact of Plant-Parasitic Nematodes on Agriculture and Methods of Control
TL;DR: The importance of plant-parasitic nematodes in agriculture and the molecular events involved in plant-nematode interactions are highlighted.
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