Resistant and susceptible responses in tomato to cyst nematode are differentially regulated by salicylic acid.
TLDR
It is found that PR-1(P4) was a hallmark for the cultivar resistance conferred by Hero A against PCN and that nematode parasitism resulted in the inhibition of the SA signaling pathway in the susceptible cultivars.Abstract:
To understand the machinery underlying a tomato cultivar harboring the Hero A gene against cyst nematode using microarrays, we fi rst analyzed tomato gene expression in response to potato cyst nematode (PCN; Globodera rostochiensis ) during the early incompatible and compatible interactions at 3 and 7 days post-inoculation (dpi). Transcript levels of the phenylalanine ammonia lyase ( PAL ) and Myb related genes were up-regulated at 3 dpi in the incompatible interaction. Transcription of the genes encoding pyruvate decarboxylase ( PDC ) and alcohol dehydrogenase ( ADH ) was also up-regulated at 3 dpi in the incompatible interaction. On the other hand, the four genes ( PAL , Myb , PDC and ADH ) were down-regulated in the compatible interaction at 3 dpi. When the expression levels of several pathogenesis-related (PR) protein genes in tomato roots were compared between the incompatible and compatible interactions, the salicylic acid (SA)-dependent PR genes were found to be induced in the incompatible interaction at 3 dpi. The PR-1 ( P4 ) transcript increased to an exceptionally high level at 3 dpi in the cyst nematode-infected resistant plants compared with the uninoculated controls. The free SA levels were elevated to similar levels in both incompatible and compatible interactions. We then confi rmed that PR-1 ( P4 ) was not signifi cantly induced in the NahG tomato harboring the Hero A gene, compared with the resistant cultivar. We thus found that PR-1 ( P4 ) was a hallmark for the cultivar resistance conferred by Hero A against PCN and that nematode parasitism resulted in the inhibition of the SA signaling pathway in the susceptible cultivars.read more
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TOMATOMA : A Novel Tomato Mutant Database Distributing Micro-Tom Mutant Collections
Takeshi Saito,Tohru Ariizumi,Yoshihiro Okabe,Erika Asamizu,Kyoko Hiwasa-Tanase,Naoya Fukuda,Tsuyoshi Mizoguchi,Yukiko Yamazaki,Koh Aoki,Hiroshi Ezura +9 more
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Tomato TILLING Technology: Development of a Reverse Genetics Tool for the Efficient Isolation of Mutants from Micro-Tom Mutant Libraries
Yoshihiro Okabe,Erika Asamizu,Takeshi Saito,Chiaki Matsukura,Tohru Ariizumi,Cécile Bres,Christophe Rothan,Tsuyoshi Mizoguchi,Hiroshi Ezura +8 more
TL;DR: Two allelic mutants of SlETR1 (Sletr 1-1 and Sletr1-2) that resulted in reduced ethylene responses were identified, indicating that the Micro-Tom TILLING platform provides a powerful tool for the rapid detection of mutations in an EMS mutant library.
Journal ArticleDOI
The endophytic strain Fusarium oxysporum Fo47: a good candidate for priming the defense responses in tomato roots.
TL;DR: It is demonstrated that priming of tomato defense responses is one of the mechanisms of action of Fo47, which induces a reduced colonization of the root of the tomato plant preinoculated with Fo47.
Journal ArticleDOI
Expression of tomato salicylic acid (SA)-responsive pathogenesis-related genes in Mi-1-mediated and SA-induced resistance to root-knot nematodes.
TL;DR: The data presented in this study show that the repression of host defence SA signalling is associated with the successful development of R KNs, and that SA exogenously added as a soil drench is able to trigger a SAR-like response to RKNs in tomato.
Book ChapterDOI
The Impact of Plant-Parasitic Nematodes on Agriculture and Methods of Control
TL;DR: The importance of plant-parasitic nematodes in agriculture and the molecular events involved in plant-nematode interactions are highlighted.
References
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Journal ArticleDOI
The broad‐spectrum potato cyst nematode resistance gene (Hero) from tomato is the only member of a large gene family of NBS‐LRR genes with an unusual amino acid repeat in the LRR region
TL;DR: The Hero gene of tomato is a broad spectrum resistance gene that confers a high level of resistance to all pathotypes of the potato cyst nematodes Globodera rostochiensis and partial resistance to G. pallida.
Journal ArticleDOI
Isolation and characterization of a tobacco mosaic virus-inducible myb oncogene homolog from tobacco
Yinong Yang,Daniel F. Klessig +1 more
TL;DR: Results suggest that the tobacco myb1 gene encodes a signaling component down-stream of SA that may participate in transcriptional activation of PR genes and plant disease resistance.
Journal ArticleDOI
Tomato Susceptibility to Root-Knot Nematodes Requires an Intact Jasmonic Acid Signaling Pathway
Kishor Bhattarai,Qi-Guang Xie,Sophie Mantelin,Usha Bishnoi,Thomas Girke,Duroy A. Navarre,Isgouhi Kaloshian +6 more
TL;DR: Results indicate that JA-dependent signaling does not play a role in Mi-1-mediated defense; however, an intact JA signaling pathway is required for tomato susceptibility to RKNs.
Journal ArticleDOI
A Genomic Cluster Containing Four Differentially Regulated Subtilisin-like Processing Protease Genes Is in Tomato Plants
TL;DR: It is proposed that these four P69-like proteases, as members of a complex gene family of plant subtilisin-like proteins, may be involved in a number of specific proteolytic events that occur in the plant during development and/or pathogenesis.
Journal ArticleDOI
Map-based cloning of a gene sequence encoding a nucleotide-binding domain and a leucine-rich region at the Cre3 nematode resistance locus of wheat
TL;DR: The Cre3 gene confers a high level of resistance to the root endoparasitic nematode Heterodera avenae in wheat and two related gene sequences have been analysed at the Cre3 locus, which appears to be a pseudogene with a frame shift caused by a deletion event.