Journal ArticleDOI
Ribosomal proteins: VI. Preparative polyacrylamide del electrophoresis as applied to the isolation of ribosomal proteins.
E. Kaltschmidt,H.G. Wittmann +1 more
TLDR
A new technique for the isolation of single proteins from complex protein mixtures by means of electrophoresis in polyacrylamide gels is described, which allows the resolution of multicomponent mixtures containing a variety of proteins which differ partly only slightly in electrophoretic mobility from each other.About:
This article is published in Analytical Biochemistry.The article was published on 1969-07-01. It has received 26 citations till now. The article focuses on the topics: Ribosomal protein & Gel electrophoresis.read more
Citations
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Journal ArticleDOI
Ribosomal proteins. Isolation of the proteins from 30S ribosomal subunits of Escherichia coli.
Journal ArticleDOI
Ribosomal proteins. Isolation of proteins from 50S ribosomal subunits of Escherichia coli.
Journal ArticleDOI
An Attempt at the Identification of the Proteins Involved in the Incorporation of 5‐S RNA during 50‐S Ribosomal Subunit Assembly
TL;DR: A simple and specific system in vitro which reflects some of the intricacies of the Escherichia coli 50-S subunit is developed and the formation of a stable complex which contains 5-S RNA and 23-S RNAs and which is dependent upon only two or three ribosomal proteins.
Journal ArticleDOI
Separation of EDTA-extractable erythrocyte membrane proteins by isoelectric focussing linked to electrophoresis in sodium dodecyl sulfate
TL;DR: The separation techniques presented here provide greater resolution than hitherto attained and should prove useful for the detailed analysis and characterisation of membrane proteins.
Journal ArticleDOI
Eukaryotic ribosomal proteins. Two-dimensional electrophoretic studies.
TL;DR: Evidence is reported below which indicates, as expected, that ribosomes from two species differ in some of their protein components, but unexpectedly, some differences are also found in two different organs in the same species.
References
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Journal ArticleDOI
Disk Electrophoresis of Basic Proteins and Peptides on Polyacrylamide Gels
TL;DR: DISK electrophoresis on small columns of polyacrylamide gels, a new method for the separation of serum proteins, has been developed by Ornstein and Davis1,2.
Journal ArticleDOI
An apparatus for preparative temperature-regulated polyacrylamide gel electrophoresis.
TL;DR: An apparatus suitable for preparative temperature-regulated polyacrylamide gel electrophoresis in discontinuous buffer systems is described and preliminary application to enzymes has demonstrated the feasibility of purification and recovery of activity.
Journal ArticleDOI
Preparative zone electrophoresis of proteins on polyacrylamide gels in 8 M urea.
P.H. Duesberg,R.R. Rueckert +1 more
TL;DR: The design and operation of an apparatus for preparative electrophoretic resolution of proteins is described and it is anticipated that the method will be particularly useful for purification of macromolecules soluble in urea or nonionic detergents, but not in the usual aqueous buffers.
Journal ArticleDOI
Preparative methods for disk electrophoresis with special reference to the isolation of pituitary hormones.
U.J. Lewis,M.O. Clark +1 more
TL;DR: The design and use of several devices which can be used for the isolation of proteins by the disk electrophoretic technique are described, including means of accurately and reproducibly sectioning columns of polyacrylamide, and a preparative device for collecting protein components as they emerge from the end of an electrophic column.
Related Papers (5)
Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.
E. Kaltschmidt,H.G. Wittmann +1 more
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
Ribosomal Proteins, XII. Number of Proteins in Small and Large Ribosomal Subunits of Escherichia coli as Determined by Two-Dimensional Gel Electrophoresis
E. Kaltschmidt,H. G. Wittmann +1 more