Journal ArticleDOI
Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.
E. Kaltschmidt,H.G. Wittmann +1 more
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TLDR
A two-dimensional polyacrylamide gel system has been developed, improving the analytical separation of complex protein mixtures as obtained from ribosomes, and facilitates and accelerates considerably a number of investigations on the structure of ribosome.About:
This article is published in Analytical Biochemistry.The article was published on 1970-08-01. It has received 1050 citations till now. The article focuses on the topics: Ribosomal protein & Polyacrylamide gel electrophoresis.read more
Citations
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Journal ArticleDOI
High resolution two-dimensional electrophoresis of proteins.
TL;DR: This technique provides a method for estimation of the number of proteins made by any biological system and can resolve proteins differing in a single charge and consequently can be used in the analysis of in vivo modifications resulting in a change in charge.
Journal ArticleDOI
High resolution two-dimensional electrophoresis of basic as well as acidic proteins
TL;DR: This paper describes an alternate procedure for the first dimension which, unlike isoelectric focusing, resolves basic as well as acidic proteins, and involves a short time of electrophoresis toward the cathode and separates most proteins according to their isoelection points.
Book ChapterDOI
Polyacrylamide Gel Electrophoresis of Viral Proteins
TL;DR: This chapter describes polyacrylamide gel electrophoresis of viral proteins in gels, which are extensively used at the present time and are more homogeneous and stable than other commonly used materials.
Journal ArticleDOI
Protein blotting: Principles and applications
Jonathan M. Gershoni,G E Palade +1 more
TL;DR: Twenty-eight years ago, Smithies demonstrated that a starch gel could serve as a molecular sieve through which zone electrophoresis of proteins could be carried out and this procedure is now one of the most widely used analytical and preparative tools in cellular and molecular biology.
Journal ArticleDOI
Micro-sequence analysis of peptides and proteins using 4-NN-dimethylaminoazobenzene 4′-isothiocyanate/phenylisothiocyanate double coupling method
TL;DR: The pheny~sot~ocyanate (Edman) degradation has been a most valuable technique in amino acid sequence determination and contributed, to a great extent, to the sequence analysis of proteins which are available in only limited quantities.
References
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Journal ArticleDOI
The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins.
Journal ArticleDOI
Acrylamide gel electrophoresis.
TL;DR: It is, however, beyond the scope of this paper to consider the theoretical problems of electrophoresis as modified by the filtration effect, and this paper is limited to the applications of gel electrophoreis and to the problems the authors have encountered with it since its first publication in 1959.
Journal ArticleDOI
The requirements for specific sRNA binding by ribosomes.
TL;DR: It is concluded that the structural properties of the sRNA, mRNA and the ribosomes are sufficient per se to determine the specificity of their interaction.
Journal ArticleDOI
Ribosomal proteins of bacterial cells: strain- and species-specificity.
TL;DR: It is revealed that the 50 and 30 s subunits which contain respectively at least 15 and 11 main protein components possess almost no common protein components.
Journal ArticleDOI
Two-dimensional Resolution of Plasma Proteins by Combination of Polyacrylamide Disc and Gradient Gel Electrophoresis
J. Margolis,K.G. Kenrick +1 more
TL;DR: Gradient gels are studied in which protein molecules are driven through progressively decreasing pores until they are brought to a near dead stop in order of their size; a procedure appropriately called “pore-limit electrophoresis”5.