Saccharomyces cerevisiae: a sexy yeast with a prion problem.
Amy C. Kelly,Reed B. Wickner +1 more
TLDR
The estimate of outcrossing suggests that Saccharomyces cerevisiae is far more sexual than previously thought and would therefore be more responsive to the adaptive effects of natural selection compared with a strictly asexual yeast.Abstract:
Yeast prions are infectious proteins that spread exclusively by mating. The frequency of prions in the wild therefore largely reflects the rate of spread by mating counterbalanced by prion growth slowing effects in the host. We recently showed that the frequency of outcross mating is about 1% of mitotic doublings with 23–46% of total matings being outcrosses. These findings imply that even the mildest forms of the [PSI+], [URE3] and [PIN+] prions impart > 1% growth/survival detriment on their hosts. Our estimate of outcrossing suggests that Saccharomyces cerevisiae is far more sexual than previously thought and would therefore be more responsive to the adaptive effects of natural selection compared with a strictly asexual yeast. Further, given its large effective population size, a growth/survival detriment of > 1% for yeast prions should strongly select against prion-infected strains in wild populations of Saccharomyces cerevisiae.read more
Citations
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References
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Journal ArticleDOI
Evolution in Mendelian Populations.
TL;DR: Page 108, last line of text, for "P/P″" read "P′/ P″."
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Evolution in Mendelian populations
TL;DR: The frequency of a given gene in a population may be modified by a number of conditions including recurrent mutation to and from it, migration, selection of various sorts and, far from least in importance, were chance variation.
Journal ArticleDOI
Population genomics of domestic and wild yeasts
Gianni Liti,David M. Carter,Alan M. Moses,Alan M. Moses,Jonas Warringer,Leopold Parts,Stephen A. James,Robert P. Davey,Ian N. Roberts,Austin Burt,Vassiliki Koufopanou,Isheng J. Tsai,Casey M. Bergman,Douda Bensasson,Michael J. T. O’Kelly,Alexander van Oudenaarden,David B. H. Barton,Elizabeth Bailes,Alex N. Nguyen,Matthew Jones,Michael A. Quail,Ian Goodhead,Ian Goodhead,Sarah Sims,Frances Smith,Anders Blomberg,Richard Durbin,Edward J. Louis +27 more
TL;DR: Rather than one or two domestication events leading to the extant baker’s yeasts, the population structure of S. cerevisiae consists of a few well-defined, geographically isolated lineages and many different mosaics of these lineages, supporting the idea that human influence provided the opportunity for cross-breeding and production of new combinations of pre-existing variations.
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Evaluation of methods for detecting recombination from DNA sequences: Computer simulations
David Posada,Keith A. Crandall +1 more
TL;DR: Methods that use substitution patterns or incompatibility among sites were more powerful than methods based on phylogenetic incongruence, and different recombination methods showed distinct performance depending on the amount of recombination, genetic diversity, and rate variation among sites.
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[URE3] as an altered URE2 protein: evidence for a prion analog in Saccharomyces cerevisiae.
TL;DR: The genetic evidence presented here suggests that protein-based inheritance, involving a protein unrelated to the mammalian prion protein, can occur in a microorganism.