Selecting for lactic acid producing and utilising bacteria in anaerobic enrichment cultures
Julius L Rombouts,Elsemiek Madeleine Maria Kranendonk,Alberte Regueira,David G. Weissbrodt,Robbert Kleerebezem,Mark C.M. Van Loosdrecht +5 more
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TLDR
This study underlines that the competitive advantage for lactic acid‐producing bacteria primarily lies in their ability to attain a high biomass specific uptake rate of glucose, which was two times higher for the complex medium enrichment when compared to the mineral medium enrichment.Abstract:
Lactic acid-producing bacteria are important in many fermentations, such as the production of biobased plastics. Insight in the competitive advantage of lactic acid bacteria over other fermentative bacteria in a mixed culture enables ecology-based process design and can aid the development of sustainable and energy-efficient bioprocesses. Here we demonstrate the enrichment of lactic acid bacteria in a controlled sequencing batch bioreactor environment using a glucose-based medium supplemented with peptides and B vitamins. A mineral medium enrichment operated in parallel was dominated by Ethanoligenens species and fermented glucose to acetate, butyrate and hydrogen. The complex medium enrichment was populated by Lactococcus, Lactobacillus and Megasphaera species and showed a product spectrum of acetate, ethanol, propionate, butyrate and valerate. An intermediate peak of lactate was observed, showing the simultaneous production and consumption of lactate, which is of concern for lactic acid production purposes. This study underlines that the competitive advantage for lactic acid-producing bacteria primarily lies in their ability to attain a high biomass specific uptake rate of glucose, which was two times higher for the complex medium enrichment when compared to the mineral medium enrichment. The competitive advantage of lactic acid production in rich media can be explained using a resource allocation theory for microbial growth processes.read more
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A review on the factors influencing biohydrogen production from lactate: The key to unlocking enhanced dark fermentative processes.
Octavio García-Depraect,Roberto Castro-Muñoz,Raúl Muñoz,Eldon R. Rene,Elizabeth León-Becerril,Idania Valdez-Vazquez,Gopalakrishnan Kumar,Luis C. Reyes-Alvarado,Leonardo J. Martínez-Mendoza,Julián Carrillo-Reyes,Germán Buitrón +10 more
TL;DR: In this paper, the authors discuss the key factors influencing the lactate-driven hydrogen production, paying particular attention to substrate composition, the operating conditions, as well as the microbiota involved in the process and its potential functionality and related biochemical routes.
Journal ArticleDOI
Organic acids production from lactic acid bacteria: A preservation approach
TL;DR: In this article , the potential of lactic acid bacteria (LAB) as a biological catalyst for producing different organic acids has been discussed and a critical snapshot of the strategies to improve microbial organic acid production and potential application of organic acids is discussed.
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Dynamics of dark fermentation microbial communities in the light of lactate and butyrate production.
Anna Detman,Daniel Laubitz,Aleksandra Chojnacka,Aleksandra Chojnacka,Pawel R. Kiela,Agnieszka Salamon,Albert Barberán,Yongjian Chen,Fei Yang,Mieczysław Błaszczyk,Anna Sikora +10 more
TL;DR: The dynamics of metabolic activity and composition of DF-MCs dependent on fermentation conditions were revealed and the pH values were shown to be the most relevant for the process of lactate and acetate conversion to butyrate.
Journal ArticleDOI
Volatile Fatty Acid Production from Organic Waste with the Emphasis on Membrane-Based Recovery
TL;DR: In this article, the authors comprehensively summarized VFA production, the factors affecting VFAs production, and VFA recovery strategies using membrane-based techniques, and the outlook for future research on VFO production is discussed.
Journal ArticleDOI
Biohydrogen production by mixed culture of Megasphaera elsdenii with lactic acid bacteria as Lactate-driven dark fermentation.
TL;DR: In this paper, the utility of Megasphaera elsdenii and LAB co-culturing in lactate-driven DF was evaluated, and the authors achieved a stable hydrogen yield of 0.95-1.49 H2-mol/mol-glucose.
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