Journal ArticleDOI
Sequence and functional expression of the GABA A receptor shows a ligand-gated receptor super-family
Peter R. Schofield,Peter R. Schofield,Mark G. Darlison,Norihisa Fujita,David R. Burt,David R. Burt,F. Anne Stephenson,Henry Rodriguez,Lucy Rhee,J. Ramachandran,V. Reale,TA Glencorse,Peter H. Seeburg,Peter H. Seeburg,Eric A. Barnard +14 more
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TLDR
Amino-acid sequences derived from complementary DMAs encoding the α- and β-subunits of the GAB A/ benzo diazepine receptor from bovine brain show homology with other ligand-gated receptor subunits, suggesting that there is a super-family of ion-channel-containing receptors.Abstract:
Amino-acid sequences derived from complementary DMAs encoding the α- and β-subunits of the GAB A/ benzo diazepine receptor from bovine brain show homology with other ligand-gated receptor subunits, suggesting that there is a super-family of ion-channel-containing receptors. Co-expression of the in vitro-generated α-subunit and β-subunit RNAs in Xenopus oocytes produces a functional receptor and ion channel with the pharmacological properties characteristic of the GABAA receptor.read more
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Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA.
John R. Riordan,Johanna M. Rommens,Batsheva Kerem,Noa Alon,Richard Rozmahel,Zbyszko Grzelczak,Julian Zielenski,Si Lok,Natasa Plavsic,Jia Ling Chou,Mitchell L. Drumm,Michael C. Iannuzzi,Francis S. Collins,Lap-Chee Tsui +13 more
TL;DR: A deletion of three base pairs that results in the omission of a phenylalanine residue at the center of the first predicted nucleotide-binding domain was detected in CF patients.
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GABAA Receptor Channels
TL;DR: This chapter discusses the gamma-aminobutyric acid (GABA) receptor channels, which are the most abundant inhibitory neurotransmitter in the CNS.
Journal ArticleDOI
A family of AMPA-selective glutamate receptors
Kari Keinänen,William Wisden,Bernd Sommer,Pia Werner,Anne Herb,Todd A. Verdoorn,Bert Sakmann,Peter H. Seeburg +7 more
TL;DR: Four cloned cDNAs encoding 900-amino acid putative glutamate receptors with approximately 70 percent sequence identity were isolated from a rat brain cDNA library and in situ hybridization revealed differential expression patterns of the cognate mRNAs throughout the brain.
Journal ArticleDOI
Requirement for glycine in activation of NMDA-receptors expressed in Xenopus oocytes
TL;DR: In voltage-clamped oocytes, neither perfusion nor rapid pressure application of NMDA onto messenger RNA-injected oocytes caused a distinct ionic current without added glycine, but when glycine was added, NMDA evoked large inward currents.
Journal ArticleDOI
Importance of a novel GABAA receptor subunit for benzodiazepine pharmacology.
Dolan B. Pritchett,Harald Sontheimer,Brenda D. Shivers,Sanie Ymer,Helmut Kettenmann,Peter R. Schofield,Peter H. Seeburg +6 more
TL;DR: The isolation of a cloned cDNA encoding a new GABAA receptor subunit, termed γ2, which shares approximately 40% sequence identity with α-and β-subunits and whose messenger RNA is prominently localized in neuronal subpopulations throughout the CNS.