Journal ArticleDOI
Serum levels of IL-8, IFNγ, IL-10, and TGF β and their gene expression levels in severe and non-severe cases of dengue virus infection
TLDR
Evidence is provided of an association of IL-8, IFNγ, TGF β and IL-10 levels with the severity of dengue illness and how these levels can be used as a predictor of severe DV infection.Abstract:
Increased serum and mRNA levels of cytokines in patients with dengue virus (DV) infection suggest that cytokines are one of the key factors in the pathogenesis of disease caused by this virus. Here, we tested 211 serum and 56 mRNA samples from an equal number of dengue cases to determine the levels of interleukin-8 (IL-8), interferon gamma (IFN-γ), interleukin-10 (IL-10) and transforming growth factor beta (TGF-β). A total 70 serum and 15 mRNA samples from healthy individual were also tested for cytokines and served as controls. Serum and mRNA levels of IL-8 were highest in the earlier days of dengue infection. IFNγ levels peaked one or two days before defervescence. Levels of IL-10 and TGF-β were highest later in dengue infection, and TGF-β levels peaked on the day of defervescence. Mean levels of IFNγ, TGF β and IL-10 were higher in samples from dengue cases, irrespective of severity, than in healthy controls. In contrast, the level of IL-8 was significantly higher in samples from severe dengue cases and lower in cases of dengue without warning signs than in healthy controls. Children (82.2 % of 101 paediatric cases) commonly had severe dengue illness. Samples that were positive for anti-DV IgG antibody had higher levels of IL-8 and TGF β. DV-2 infections were associated with severe dengue illness. IL-8 and IFNγ levels were higher in the presence of warning signs of severe dengue. Levels of IL-8, IL-10 and TGF β were independently associated with disease outcome. These data provide evidence of an association of IL-8, IFNγ, TGF β and IL-10 levels with the severity of dengue illness. Especially, IL-8 levels can be used as a predictor of severe DV infection.read more
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Journal ArticleDOI
Zika viral dynamics and shedding in rhesus and cynomolgus macaques.
Christa E. Osuna,So-Yon Lim,Claire Deleage,Bryan D. Griffin,Derek R. Stein,Lukas T. Schroeder,Robert W Omange,Katharine Best,Ma Luo,Peter T. Hraber,Hanne Andersen-Elyard,Erwing Fabian Cardozo Ojeda,Scott Huang,Dana L. Vanlandingham,Stephen Higgs,Alan S. Perelson,Jacob D. Estes,David Safronetz,Mark G. Lewis,James B. Whitney,James B. Whitney +20 more
TL;DR: It is shown that both rhesus and cynomolgus macaques are highly susceptible to infection by lineages of Zika virus that are closely related to, or are currently circulating in, the Americas.
Journal ArticleDOI
Viral Innate Immune Evasion and the Pathogenesis of Emerging RNA Virus Infections
Tessa Nelemans,Marjolein Kikkert +1 more
TL;DR: What is currently known about the role of interferons and host immune evasion in the pathogenesis of emerging coronaviruses, alphavirus and flaviviruses is discussed.
Journal ArticleDOI
Cytokine kinetics of Zika virus-infected patients from acute to reconvalescent phase.
Dennis Tappe,José Vicente Pérez-Girón,Lorenzo Zammarchi,Jürgen Rissland,Davis Fernandes Ferreira,Thomas Jaenisch,Sergio Gómez-Medina,Stephan Günther,Alessandro Bartoloni,César Muñoz-Fontela,César Muñoz-Fontela,Jonas Schmidt-Chanasit +11 more
TL;DR: Polyfunctional T cell activation (Th1, Th2, Th9, and Th17 response) was seen during the acute phase characterized by respective cytokine level increases, followed by a decrease in the reconvalescent phase.
Journal ArticleDOI
Serum from dengue virus-infected patients with and without plasma leakage differentially affects endothelial cells barrier function in vitro
Francielle Tramontini Gomes de Sousa Cardozo,Gyulnar Baimukanova,Marion C. Lanteri,Marion C. Lanteri,Sheila M. Keating,Sheila M. Keating,Frederico Moraes Ferreira,John W. Heitman,Cláudio Sérgio Pannuti,Shibani Pati,Camila Malta Romano,Ester Cerdeira Sabino +11 more
TL;DR: The present work shows that the magnitude of the immune response contributes to the adverse plasma leakage outcomes in patients and that serum components are important mediators of changes in endothelial homeostasis during dengue infections.
Journal ArticleDOI
Vaccines licensed and in clinical trials for the prevention of dengue
TL;DR: The chimeric yellow fever-dengue vaccine (CYD) that employs the yellow fever virus 17D strain as the replication backbone (Chimerivax-DEN; CYD-TDV) had an overall pooled protective efficacy of 65.6% but was substantially more effective against severe d Dengue and dengue hemorrhagic fever.
References
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