Journal ArticleDOI
Single myosin molecule mechanics: piconewton forces and nanometre steps
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A new in vitro assay using a feedback enhanced laser trap system allows direct measurement of force and displacement that results from the interaction of a single myosin molecule with a single suspended actin filament.Abstract:
A new in vitro assay using a feedback enhanced laser trap system allows direct measurement of force and displacement that results from the interaction of a single myosin molecule with a single suspended actin filament. Discrete stepwise movements averaging 11 nm were seen under conditions of low load, and single force transients averaging 3-4 pN were measured under isometric conditions. The magnitudes of the single forces and displacements are consistent with predictions of the conventional swinging-crossbridge model of muscle contraction.read more
Citations
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Journal ArticleDOI
Methods to find aponeurosis and tendon stiffness and the onset of muscle contraction
TL;DR: A method to measure small movements of living tissues either large or small is presented based on the detection of changes in reflected infrared light using an optocoupler and a small (< 1 cm2) mirror.
Book ChapterDOI
Optical force microscopy.
Andrea L. Stout,Watt W. Webb +1 more
TL;DR: This chapter discusses the optical force microscopy, which has become established not only as a tool for manipulating microscopic particles, but as a sensitive force transducer as well.
Proceedings ArticleDOI
Trapping force calibration in biological applications of optical tweezers
Sergey A. Ermilov,Bahman Anvari +1 more
TL;DR: In this article, the displacement of the trapped object from the trapping center using Fourier analysis of the equation of motion is calculated using the Stokes' Law based on inertia-free assumptions.
Journal ArticleDOI
Single molecule studies of homologous recombination
TL;DR: An overview of single molecule assays that have been developed to study key aspects of homologous recombination are presented and the unique information gleaned from these experiments are discussed.
Journal ArticleDOI
How Cells Feel: Stochastic Model for a Molecular Mechanosensor
TL;DR: A self-consistent physical model for mechanosensitivity is developed, a process by which a cell detects the mechanical stiffness of its environment and generates an appropriate chemical signaling to remodel itself in response to this environment, which offers the underlying mechanism for the myofibroblast conversion in wound healing and smooth muscle cell dysfunction in cardiac disease.
References
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Journal ArticleDOI
Proposed Mechanism of Force Generation in Striated Muscle
A. F. Huxley,R. M. Simmons +1 more
TL;DR: Recordings of the change in tension in striated muscle after a sudden alteration of the length have made it possible to suggest how the force between the thick and thin muscle filaments may be generated.
Journal ArticleDOI
Three-dimensional structure of myosin subfragment-1: a molecular motor
Ivan Rayment,Wojciech Rypniewski,Karen Schmidt-Bäse,Karen Schmidt-Bäse,Robert Smith,Diana R. Tomchick,Diana R. Tomchick,Matthew M. Benning,Donald A. Winkelmann,Gary E. Wesenberg,Hazel M. Holden +10 more
TL;DR: The three-dimensional structure of the head portion of myosin, or subfragment-1, which contains both the actin and nucleotide binding sites, is described, and this structure of a molecular motor was determined by single crystal x-ray diffraction.
Journal ArticleDOI
Direct observation of kinesin stepping by optical trapping interferometry
TL;DR: It is found that kinesin moves with 8-nm steps, similar to biological motors that move with regular steps.
Journal ArticleDOI
The Mechanism of Muscular Contraction
TL;DR: There is now a real possibility of solving the problem in complete detail, provided a way can be found to crystallize a recently purified globular subfragment of the myosin molecule, and some apparently paradoxical properties of the system are revealed.
Journal ArticleDOI
Bead movement by single kinesin molecules studied with optical tweezers
TL;DR: The results of this study are consistent with a model in which kinesin detaches briefly from the microtubule during a part of each mechanochemical cycle, rather than a models in whichKinesin remains bound at all times.