Journal ArticleDOI
Spectral characterization of the effect of viscosity on Fura-2 fluorescence: Excitation wavelength optimization abolishes the viscosity artifact
Reads0
Chats0
TLDR
A systematic study of the spectral characteristics of the viscosity artifact in Fura-2 based [Ca2+] measurements reveals that, by selecting excitation wavelengths approximately 10 nm longer than those routinely employed and modestly reducing excitation bandpasses, the magnitude of the artifact can be reduced to experimentally undetectable levels without greatly impairing [ Ca2+].About:
This article is published in Cell Calcium.The article was published on 1992-05-01. It has received 25 citations till now. The article focuses on the topics: Fluorescence spectrometry & Viscosity.read more
Citations
More filters
Journal ArticleDOI
Estimating intracellular calcium concentrations and buffering without wavelength ratioing.
TL;DR: The proposed method for determining intracellular free calcium concentration from single-wavelength fluorescence signals does not require independent estimates of resting [Ca(2+)] but relies on the measurement of fluorescence close to indicator saturation during an experiment, which makes it well suited to [ Ca( 2+)] indicators for which saturation can be achieved under physiological conditions.
Journal ArticleDOI
Imaging calcium concentration dynamics in small neuronal compartments.
Ryohei Yasuda,Esther A. Nimchinsky,Esther A. Nimchinsky,Volker Scheuss,Thomas A. Pologruto,Thomas A. Pologruto,Thomas G. Oertner,Bernardo L. Sabatini,Bernardo L. Sabatini,Karel Svoboda +9 more
TL;DR: Techniques that are optimized for 2-photon imaging of Ca2+ dynamics in small compartments such as dendrites and dendritic spines are described.
Journal ArticleDOI
Genetically encoded calcium indicators.
Marco Mank,Oliver Griesbeck +1 more
TL;DR: Progress on the biosensor side is closely linked with further improvements in engineering XFPs for more brightness, photostability, full maturation at 37 °C, and shifts to longer wavelengths of emission.
Journal ArticleDOI
Calcium mobilization is required for nuclear vesicle fusion in vitro: Implications for membrane traffic and IP3 receptor function
TL;DR: It is suggested that phosphoinositide signaling may regulate nuclear vesicle fusion in Xenopus egg extracts, and was biochemically distinct from, and occurred later than, the GTP gamma S-sensitive step mediated by the monomeric GTPase, ADP-ribosylation factor.
Journal ArticleDOI
Effects of temperature on calcium-sensitive fluorescent probes.
TL;DR: Temperature effects can influence reported calcium concentrations and must be taken into consideration during any investigation involving variable temperatures, and in most cases, low temperature led to prolonged fluorescence lifetimes.
References
More filters
Book
CRC Handbook of Chemistry and Physics
TL;DR: CRC handbook of chemistry and physics, CRC Handbook of Chemistry and Physics, CRC handbook as discussed by the authors, CRC Handbook for Chemistry and Physiology, CRC Handbook for Physics,
Journal ArticleDOI
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
TL;DR: A new family of highly fluorescent indicators has been synthesized for biochemical studies of the physiological role of cytosolic free Ca2+ using an 8-coordinate tetracarboxylate chelating site with stilbene chromophores that offer up to 30-fold brighter fluorescence.
Journal ArticleDOI
Assessment of Fura-2 for measurements of cytosolic free calcium
TL;DR: Many of the currently recognized limitations to the use of Fura-2 in living cells are described and certain approaches which can circumvent some of these problems are described.
Book ChapterDOI
Measurement of cytosolic free Ca2+ with quin2.
Roger Y. Tsien,Tullio Pozzan +1 more
TL;DR: The chapter compares quin2 with newer fluorescent indicators—fura-2 and indo-1—as they are much brighter in fluorescence than quin1, and allows a direct measurement of compartmentalization and heterogeneity.