Journal ArticleDOI
Sperm Deoxyribonucleic Acid Fragmentation is Increased in Poor-Quality Semen Samples and Correlates With Failed Fertilization in Intracytoplasmic Sperm Injection
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TLDR
The results suggest that when poor-quality semen samples are used for ICSI, there is a greater likelihood that some sperm selected for injection, despite appearing normal, contain fragmented DNA.About:
This article is published in The Journal of Urology.The article was published on 1998-11-01. It has received 282 citations till now. The article focuses on the topics: Intracytoplasmic sperm injection & Sperm.read more
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Role of sperm chromatin abnormalities and DNA damage in male infertility
Ashok Agarwal,Tamer M. Said +1 more
TL;DR: Screening for sperm DNA damage may provide useful information in cases of male idiopathic infertility and in those men pursuing assisted reproduction, and treatment should include methods for prevention of spermDNA damage.
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Sperm DNA fragmentation: mechanisms of origin, impact on reproductive outcome, and analysis
TL;DR: The mechanisms responsible for DNA fragmentation in human sperm, including those occurring during spermatogenesis and transport through the reproductive tract are reviewed, and how abnormal sperm could be dealt with by the oocyte and how sperm DNA abnormalities may interfere with normal embryo and fetal development are discussed.
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Origin of dna damage in ejaculated human spermatozoa
TL;DR: The possible origins of DNA damage in ejaculated human spermatozoa are discussed, how these spermutozoa arrive in the ejaculate of some men, and what consequences they may have if they succeed in their genetic project are discussed.
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The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development.
TL;DR: High loads of DNA damage measured by the Comet assay were predictive of failure of embryo development after ICSI, and it is likely that sperm with DNA damage contributed to successful fertilization and in-vitro development.
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DNA damage to spermatozoa has impacts on fertilization and pregnancy
TL;DR: The weight of evidence currently favours oxidative stress and aberrant apoptosis as the major candidates for DNA damage in the male germ line and positive results have been reported for antioxidant therapy both in vivo and in vitro.
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Journal ArticleDOI
Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte
TL;DR: Intracytoplasmic sperm injection is used to treat couples with infertility because of severely impaired sperm characteristics, and in whom IVF and SUZI had failed.
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Oxidative stress as a mediator of apoptosis
Thomas M. Buttke,Paul Sandstrom +1 more
TL;DR: Thomas Buttke and Paul Sandstrom suggest that eukaryotic cells may benefit from this perilous existence by invoking oxidative stress as a common mediator of apoptosis.
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Relation of mammalian sperm chromatin heterogeneity to fertility.
TL;DR: Flow cytometry of heated sperm nuclei revealed a significant decrease in resistance to in situ denaturation of spermatozoal DNA in samples from bulls, mice, and humans of low or questionable fertility when compared with others of high fertility.
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Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro.
TL;DR: Since extremely poor semen samples are the indication for intracytoplasmic sperm injection, there is a high likelihood that sperm with fragmented DNA may be selected by chance and used for oocyte injection, resulting in poor fertilization and/or cleavage rates.
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Oxidative stress induces apoptosis in embryonic cortical neurons.
TL;DR: It is found that glutathione depletion leads to hypercondensation and fragmentation of chromatin into spherical or irregular shapes, a morphologic signature of apoptosis, which suggests that oxidative stress can induce apoptosis in neurons.