Book ChapterDOI
Sperm nuclear dna damage in the human
TLDR
A standard semen analysis, in particular the assessment of sperm concentration and motility, should be viewed as a mandatory test for inclusion in the investigation of the infertile couple.Abstract:
The male partner is now recognized as the single most common contributor to a couple’s reason for infertility, with sperm defects accounting for around 30–50% of cases presenting to infertility clinics (Hull, 1992). A standard semen analysis, in particular the assessment of sperm concentration and motility, should therefore always be viewed as a mandatory test for inclusion in the investigation of the infertile couple (WHO, 1992; WHO,1999). A semen analysis, however, only helps to provide broad diagnostic categories.read more
Citations
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Journal ArticleDOI
The predictive value of sperm chromatin structure assay (SCSA) parameters for the outcome of intrauterine insemination, IVF and ICSI
TL;DR: Examination of the relationship between the results of sperm chromatin structure assay (SCSA) and the outcome of IVF, ICSI and intrauterine insemination concluded that SCSA is a useful method for prediction of the outcomes of assisted reproduction.
Journal ArticleDOI
Simple determination of human sperm DNA fragmentation with an improved sperm chromatin dispersion test
José L. Fernández,Lourdes Muriel,Vicente Goyanes,Enrique Segrelles,Jaime Gosálvez,María Enciso,Marie LaFromboise,Christopher J. De Jonge +7 more
TL;DR: The improved SCD protocol (Halosperm kit) results in better chromatin preservation, therefore highly contrasted halo images can be accurately assessed using conventional bright-field microscopy after Wright staining, making it possible to unequivocally discriminate sperm from other cell types.
Journal ArticleDOI
Detection of damage in mammalian sperm cells.
TL;DR: The integrity of sperm DNA can be measured at three different levels by assessing the degree of DNA-protamine condensation, the incidence of breaks and nicks in the DNA and the frequency of fragmentation of the nuclei into sub-haploid apoptotic bodies.
Journal ArticleDOI
Increased sperm DNA damage in patients with varicocele: relationship with seminal oxidative stress
Rosita Smith,Heidy Kaune,Daniela Parodi,Marcia Madariaga,Rafael Ríos,Ignacio Morales,Andrea Castro +6 more
TL;DR: The results indicate that oxidative damage is associated with sperm DNA damage in patients with varicocele and that high levels of DNA-damage spermatozoa even in the presence of normal semen profile are found.
Journal ArticleDOI
Full‐term pregnancies achieved with ICSI despite high levels of sperm chromatin damage
Loredana Gandini,Francesco Lombardo,Donatella Paoli,F. Caruso,Patrizia Eleuteri,Giorgio Leter,Rosanna Ciriminna,Franco Culasso,Franco Dondero,Andrea Lenzi,Marcello Spanò +10 more
TL;DR: There is still controversy over whether analytical techniques currently in use are able to identify the level of damage to spermatozoa and large-scale studies should be conducted in different clinical settings to determine the effects of sperm DNA damage on the outcome of ART.
References
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Journal ArticleDOI
Molecular cloning and expression of the fas ligand, a novel member of the tumor necrosis factor family
TL;DR: Northern hybridization revealed that Fas ligand is expressed in activated splenocytes and thymocytes, consistent with its involvement in T cell-mediated cytotoxicity and in several nonlymphoid tissues, such as testis.
Journal ArticleDOI
Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic
Donald P. Evenson,Lorna K. Jost,D Marshall,M J Zinaman,E D Clegg,K Purvis,P. De Angelis,O P Claussen +7 more
TL;DR: Based on logistic regression, spermatozoa with denatured DNA (cells outside the main population, COMP alpha t) were the best predictor for whether a couple would not achieve pregnancy.
Journal ArticleDOI
Relation of mammalian sperm chromatin heterogeneity to fertility.
TL;DR: Flow cytometry of heated sperm nuclei revealed a significant decrease in resistance to in situ denaturation of spermatozoal DNA in samples from bulls, mice, and humans of low or questionable fertility when compared with others of high fertility.
Journal ArticleDOI
Relative Impact of Oxidative Stress on the Functional Competence and Genomic Integrity of Human Spermatozoa
TL;DR: Human spermatozoa exposed to increasing levels of oxidative stress achieved through the stimulation of endogenous oxidant generation with NADPH or direct exposure to hydrogen peroxide exhibited significantly elevated levels of DNA damage and yet continued to express an enhanced capacity for sperm-oocyte fusion.
Journal ArticleDOI
Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro.
TL;DR: Since extremely poor semen samples are the indication for intracytoplasmic sperm injection, there is a high likelihood that sperm with fragmented DNA may be selected by chance and used for oocyte injection, resulting in poor fertilization and/or cleavage rates.
Related Papers (5)
Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques.
Role of sperm chromatin abnormalities and DNA damage in male infertility
Ashok Agarwal,Tamer M. Said +1 more