Structural determinants of Ricinus communis agglutinin and toxin specificity for oligosaccharides.
J U Baenziger,D Fiete +1 more
TLDR
Structural determinants of oligosaccharides affecting interaction with the saccharide-binding sites of two lectins, the agglutinin of M, 120,000 and toxin of 82, 60,000 (RCA* and RCA~I, respectively), isolated from Ricinus communis beans have been examined by performing binding studies with iodinated glycopeptides and their degradation products.About:
This article is published in Journal of Biological Chemistry.The article was published on 1979-10-10 and is currently open access. It has received 464 citations till now.read more
Citations
More filters
Journal ArticleDOI
Carbohydrate-binding modules: fine-tuning polysaccharide recognition
TL;DR: The present review summarizes the impact structural biology has had on the understanding of the mechanisms by which CBMs bind to their target ligands.
Journal ArticleDOI
Biological Roles of Glycans
TL;DR: It is time for the diverse functional roles of glycans to be fully incorporated into the mainstream of biological sciences, as they are no different from other major macromolecular building blocks of life, simply more rapidly evolving and complex.
Journal ArticleDOI
Specificity of Twelve Lectins Towards Oligosaccharides and Glycopeptides Related to N‐Glycosylproteins
TL;DR: The results obtained show that lectins considered 'identical' in terms of monosaccharide specificity, possess the ability to recognize fine differences in more complex structures, and different lectins are able to recognize different saccharidic sequences on the same glycan structure.
Journal ArticleDOI
The preparation and cytotoxic properties of antibody-toxin conjugates.
TL;DR: The decision to use toxins as the cytotoxic component of the conjugate was provoked by their supreme potency, and maximizes the chance of killing cancer cells which do not express specific antigens at high density or which reside in solid tumors not freely permeable to the Conjugate.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
The Attractions of Proteins for Small Molecules and Ions
TL;DR: The number and variety of known compounrjs between proteins and small molecules are increasing rapidly and make a fascinating story as discussed by the authors, and there are many compounds of serum albumin, which was used during the war by many chemists, most of whom found at least one 6ew compound.
Journal ArticleDOI
Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril.
Pamela J. Fraker,John C. Speck +1 more
TL;DR: The stability and sparing solubility of this chloroglycoluril in water can account for the minimal damage to proteins and living cells observed in these iodinations and allow for elimination of the reduction step employed at the close of iodinations with soluble chloroamides such as chloramine-T.
Journal ArticleDOI
The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Application to the radioimmunoassay
A. E. Bolton,William M. Hunter +1 more
TL;DR: With some antisera the immunoreactivity of the antigen was diminished by the introduction of a single I atom into the tyrosyl groups, whereas antigen containing a single (125)I-labelled 3-(4-hydroxyphenyl)propionamide group showed the same immunore activity as the unmodified antigen.