Journal ArticleDOI
Structure and function of pancreatic lipase and colipase
TLDR
This review discusses the advances made in protein structure and in understanding the relationships of structure to function of pancreatic triglyceride lipase and colipase.Abstract:
Dietary fats are essential for life and good health. Efficient absorption of dietary fats is dependent on the action of pancreatic triglyceride lipase. In the last few years, large advances have been made in describing the structure and lipolytic mechanism of human pancreatic triglyceride lipase and of colipase, another pancreatic protein that interacts with pancreatic triglyceride lipase and that is required for lipase activity in the duodenum. This review discusses the advances made in protein structure and in understanding the relationships of structure to function of pancreatic triglyceride lipase and colipase.read more
Citations
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Strategies to Address Low Drug Solubility in Discovery and Development
Hywel David Williams,Natalie L. Trevaskis,Susan A. Charman,Ravi Mysore Shanker,William N. Charman,Colin W. Pouton,Christopher J.H. Porter +6 more
TL;DR: The article provides an integrated and contemporary discussion of current approaches to solubility and dissolution enhancement but has been deliberately structured as a series of stand-alone sections to allow also directed access to a specific technology where required.
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Enhancing intestinal drug solubilisation using lipid-based delivery systems.
TL;DR: The mechanistic rationale which underpins the use of lipid-based delivery systems to enhance drug solubilisation is described and the available literature describing increases in oral bioavailability after the administration of lipid solution, suspension and self-emulsifying formulations is reviewed.
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The digestion of dietary triacylglycerols
Huiling Mu,Carl-Erik Høy +1 more
TL;DR: Dietary triacylglycerols are the major lipid components in the human diet and they are carriers of energy as well as important fatty acids, so the influences of the fatty acid composition and the intramolecular structure of dietary TAGs on their digestion and absorption are summarized.
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The influence of emulsion structure and stability on lipid digestion
Matt Golding,Tim J. Wooster +1 more
TL;DR: In this paper, food emulsion structure and stability has been investigated in terms of its role in lipid metabolism and metabolism, and it has been shown that food emulsions have a contributing role on lipid digestion and metabolism.
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The Metabolic Serine Hydrolases and Their Functions in Mammalian Physiology and Disease
TL;DR: A comprehensive summary that captures the state of knowledge about mammalian metabolic SHs in their entirety, including those enzymes that remain mostly or completely uncharacterized.
References
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Journal ArticleDOI
Structure of human pancreatic lipase
TL;DR: The structural results are evidence that Ser 152 is the nucleophilic residue essential for catalysis, located in the larger N-terminal domain at the C- terminal edge of a doubly wound parallel β-sheet and part of an Asp-His-Ser triad, which is chemically analogous to, but structurally different from, that in the serine proteases.
Journal ArticleDOI
A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex
Andrzej M. Brzozowski,Andrzej M. Brzozowski,Urszula Derewenda,Urszula Derewenda,Zygmunt S. Derewenda,Zygmunt S. Derewenda,Guy Dodson,David M. R. Lawson,Johan P. Turkenburg,Fredrik Björkling,B. Huge-Jensen,S. A. Patkar,L. Thim +12 more
TL;DR: It is proposed that the structure of the enzyme in this complex of R. miehei lipase with n-hexylphosphonate ethyl ester is equivalent to the activated state generated by the oil–water interface.
Journal ArticleDOI
Action de la lipase pancréatique sur les esters en émulsion
L. Sarda,P. Desnuelle +1 more
TL;DR: De considerer la lipase pancreatique comme une esterase particuliere destinee a agir sur des esters insolubles au niveau of l'interface separant ces esters de l'eau, un calcul fait intervenir the “concentration molaire interfaciale” du substrat.
Journal ArticleDOI
Interfacial activation of the lipase-procolipase complex by mixed micelles revealed by X-ray crystallography.
TL;DR: The three-dimensional structure of the lipase–-procolipase complex, co-crystallized with mixed micelles of phosphatidylcholine and bile salt, has been determined at 3 Å resolution by X-ray crystallography.
Journal ArticleDOI
Relationship between sequence conservation and three‐dimensional structure in a large family of esterases, lipases, and related proteins
Miroslaw Cygler,Joseph D. Schrag,Joel L. Sussman,Michal Harel,Israel Silman,Mary K. Gentry,Bhupendra P. Doctor +6 more
TL;DR: An improved alignment of a collection of 32 related amino acid sequences of other esterases, lipases, and related proteins was obtained, and 24 residues are found to be invariant in 29 sequences of hydrolytic enzymes, and an additional 49 are well conserved.