Structure and Receptor Specificity of the Hemagglutinin from an H5N1 Influenza Virus
James Stevens,Ola Blixt,Terrence M. Tumpey,Jeffery K. Taubenberger,James C. Paulson,Ian A. Wilson +5 more
TLDR
The hemagglutinin structure at 2.9 angstrom resolution, from a highly pathogenic Vietnamese H5N1 influenza virus, is more related to the 1918 and other human H1 HAs than to a 1997 duck H5 HA, which suggests a path for this H 5N1 virus to gain a foothold in the human population.Abstract:
The hemagglutinin (HA) structure at 2.9 angstrom resolution, from a highly pathogenic Vietnamese H5N1 influenza virus, is more related to the 1918 and other human H1 HAs than to a 1997 duck H5 HA. Glycan microarray analysis of this Viet04 HA reveals an avian α2-3 sialic acid receptor binding preference. Introduction of mutations that can convert H1 serotype HAs to human α2-6 receptor specificity only enhanced or reduced affinity for avian-type receptors. However, mutations that can convert avian H2 and H3 HAs to human receptor specificity, when inserted onto the Viet04 H5 HA framework, permitted binding to a natural human α2-6 glycan, which suggests a path for this H5N1 virus to gain a foothold in the human population.read more
Citations
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疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
Journal ArticleDOI
Biological Roles of Glycans
TL;DR: It is time for the diverse functional roles of glycans to be fully incorporated into the mainstream of biological sciences, as they are no different from other major macromolecular building blocks of life, simply more rapidly evolving and complex.
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Emergence and pandemic potential of swine-origin H1N1 influenza virus.
TL;DR: Efforts to control these outbreaks and real-time monitoring of the evolution of this virus should provide invaluable information to direct infectious disease control programmes and to improve understanding of the factors that determine viral pathogenicity and/or transmissibility.
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Antibody Recognition of a Highly Conserved Influenza Virus Epitope
Damian C. Ekiert,Gira Bhabha,Marc-André Elsliger,Robert H. E. Friesen,Mandy Jongeneelen,Mark Throsby,Jaap Goudsmit,Ian A. Wilson +7 more
TL;DR: TheCR6261 epitope identified here should accelerate the design and implementation of improved vaccines that can elicit CR6261-like antibodies, as well as antibody-based therapies for the treatment of influenza.
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Experimental adaptation of an influenza H5 HA confers respiratory droplet transmission to a reassortant H5 HA/H1N1 virus in ferrets
Masaki Imai,Tokiko Watanabe,Masato Hatta,Subash C. Das,Makoto Ozawa,Makoto Ozawa,Kyoko Shinya,Gongxun Zhong,Anthony Hanson,Hiroaki Katsura,Shinji Watanabe,Chengjun Li,Eiryo Kawakami,Shinya Yamada,Maki Kiso,Yasuo Suzuki,Eileen A. Maher,Gabriele Neumann,Yoshihiro Kawaoka +18 more
TL;DR: Results indicate that H5 HA can convert to an HA that supports efficient viral transmission in mammals, and will help individuals conducting surveillance in regions with circulating H5N1 viruses to recognize key residues that predict the pandemic potential of isolate, which will inform the development, production and distribution of effective countermeasures.
References
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疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
Journal ArticleDOI
Receptor Binding and Membrane Fusion in Virus Entry: The Influenza Hemagglutinin
John J. Skehel,Don C. Wiley +1 more
TL;DR: Comparisons to the soluble N-ethyl-maleimide-sensitive factor attachment protein receptor (SNARE) protein complex of vesicle fusion suggests that these molecules are all in the fusion-activated conformation and that the juxtaposition of the membrane anchor and fusion peptide, a recurring feature, is involved in the fused mechanism.
Journal ArticleDOI
Updating the Accounts: Global Mortality of the 1918-1920 "Spanish" Influenza Pandemic
Niall Johnson,Juergen Mueller +1 more
TL;DR: The estimated global mortality of the pandemic was of the order of 50 million, and it must be acknowledged that even this vast figure may be substantially lower than the real toll, perhaps as much as 100 percent understated.
Journal ArticleDOI
Characterization of a Novel Influenza A Virus Hemagglutinin Subtype (H16) Obtained from Black-Headed Gulls
Ron A. M. Fouchier,Vincent J. Munster,Anders Wallensten,Theo M. Bestebroer,Sander Herfst,Derek J. Smith,Derek J. Smith,Gus F. Rimmelzwaan,Bjorn R. Olsen,Albert D. M. E. Osterhaus +9 more
TL;DR: A previously unidentified antigenic subtype of HA (H16), detected in viruses circulating in black-headed gulls in Sweden, is described and proposed that sequence analyses of HA and NA genes of influenza A viruses be used for the rapid identification of existing and novel HA andNA subtypes.
Journal ArticleDOI
Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation
TL;DR: Four ‘antigenic sites’ on the three-dimensional structure of the influenza haemagglutinin are identified and at least one amino acid substitution in each site seems to be required for the production of new epidemic strains between 1968 and 1975.
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