Studies of gonadotropin-releasing hormone (GnRH) action using GnRH receptor-expressing pituitary cell lines.
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GnRH Action in GH3 cells transfected with the GnRH Receptor (GGH3 cells) and regulation of GnRHR regulation are described.Abstract:
I. Introduction II. GnRHR Structure Analysis III. Studies of GnRH Action in αT3-1 Cells A. Derivation of αT3-1 cells B. Characterization of αT3-1 cells C. GnRH binding D. GnRHR regulation 1. Homologous regulation by GnRH 2. Regulation by gonadal steroid hormones 3. Regulation by gonadal peptides 4. Regulation by second messenger activators E. Intracellular second messengers 1. G protein coupling 2. Inositol phosphates 3. Intracellular calcium 4. Protein kinase C 5. cAMP 6. Mitogen-activated protein kinases F. α-Subunit gene expression 1. Cell-specific expression 2. GnRH-stimulated expression G. Desensitization H. Summary of GnRH action in αT3-1 cells IV. Studies of GnRH Action in GH3 Cells Transfected with the GnRH Receptor (GGH3 Cells) A. Derivation of GH3 cells B. Characterization of GH3 cells C. Derivation of GH3 cells transfected with the GnRHR (GGH3 cells) D. GnRH binding E. GnRHR regulation F. Intracellular second messengers 1. G protein coupling 2. Inositol phosphates 3. cAMP G. Regulation of secre...read more
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Gonadotropin-Releasing Hormone Receptors
Robert P. Millar,Robert P. Millar,Zhi-Liang Lu,Adam J. Pawson,Colleen A. Flanagan,Kevin Morgan,Stuart Maudsley +6 more
TL;DR: Delineation of the structural elements in GnRH and the receptor, which facilitate differential signaling, will contribute to the development of novel interventive GnRH analogs.
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Gonadotropin-releasing-hormone-receptor antagonists
TL;DR: In assisted reproduction, these compounds seem to be as effective as established therapy but with shorter treatment times, less use of gonadotropic hormones, improved patient acceptance, and fewer follicles and oocytes.
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Egr-1 Is a Downstream Effector of GnRH and Synergizes by Direct Interaction with Ptx1 and SF-1 To Enhance Luteinizing Hormone β Gene Transcription
TL;DR: Egr-1 is a likely transcriptional mediator of GnRH-induced signals for activation of the LHβ gene, and is implicated in key factors involved in luteinizing hormone β (LHβ) gonadotropin gene transcription.
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GnRH pulsatility, the pituitary response and reproductive dysfunction.
TL;DR: Fertility is impaired when GnRH pulsatility is inhibited by chronic malnutrition, excessive caloric expenditure, or aging and the molecular mechanisms of this pulsatile GnRH regulation are not well understood.
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Ion Channels and Signaling in the Pituitary Gland
TL;DR: This review summarizes recent findings in this field and current understanding of the complex relationship between voltage-gated ion channels, ligand- gated ion channel channels, gap junction channels, and G protein-coupled receptors in pituitary cells.
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