Synthesis of polyhydroxyalkanoate in the peroxisome of Pichia pastoris
TLDR
P. pastoris could be used as a model system to study how peroxisomal metabolism needs to be modified to increase PHA production in other eukaryotes, such as plants.Abstract:
Polyhydroxyalkanoates (PHAs) are polyesters naturally produced by bacteria that have properties of biodegradable plastics and elastomers. A PHA synthase from Pseudomonas aeruginosa modified at the carboxy-end for peroxisomal targeting was transformed in Pichia pastoris. The PHA synthase was expressed under the control of the promoter of the P. pastoris acyl-CoA oxidase gene. Synthesis of up to 1% medium-chain-length PHA per g dry weight was dependent on both the expression of the PHA synthase and the presence of oleic acid in the medium. PHA accumulated as inclusions within the peroxisomes. P. pastoris could be used as a model system to study how peroxisomal metabolism needs to be modified to increase PHA production in other eukaryotes, such as plants.read more
Citations
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Biotechnological approaches for the production of polyhydroxyalkanoates in microorganisms and plants — A review
TL;DR: Although excellent progress has been made in recombinant hosts, the barriers to obtaining high quantities of PHA at low cost still remain to be solved, and the commercially viable production of P HA in crops appears to be a realistic goal for the future.
Journal ArticleDOI
Metabolic engineering and pathway construction for biotechnological production of relevant polyhydroxyalkanoates in microorganisms
TL;DR: This review will focus on the engineering of pathways resulting in the formation of PHAs containing 3-hydroxyvaleric acid, medium-chain-length 3-Hydroxyalkanoic acids or 4-hydroxbutyric acid as constituents.
Journal ArticleDOI
An overview of lipid metabolism in yeasts and its impact on biotechnological processes.
TL;DR: This review focuses on the ongoing research on different oleaginous yeasts producing high added value lipids and on the prospects of such microbial oils to be used in different biotechnological processes and applications.
Journal ArticleDOI
Mini-Review: Biosynthesis of Poly(hydroxyalkanoates)
TL;DR: This review covers known biosynthetic pathways for the production of PHAs, which have been produced for use as bulk commodity plastics, fishing lines, and medical uses and attracted much attention as biodegradable polymers that can be produced from biorenewable resources.
Journal ArticleDOI
Metabolic engineering of Pichia pastoris.
TL;DR: The impressive methodological developments of the last years to model and analyze the metabolism of P. pastoris, and to engineer its genome and metabolic pathways are summarized.
References
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Journal ArticleDOI
Formation of Polyesters by Pseudomonas oleovorans: Effect of Substrates on Formation and Composition of Poly-(R)-3-Hydroxyalkanoates and Poly-(R)-3-Hydroxyalkenoates
Roland Gerhard Lageveen,Gjalt W. Huisman,Hans Preusting,Peter E.F. Ketelaar,G. Eggink,Bernard Witholt +5 more
TL;DR: It is shown that the intermediate 3-Hydroxy fatty acids can also be polymerized to intracellular poly-(R)-3-hydroxyalkanoates (PHAs) when the medium contains limiting amounts of essential elements, such as nitrogen.
Journal ArticleDOI
Production of polyhydroxyalkanoates, a family of biodegradable plastics and elastomers, in bacteria and plants.
TL;DR: Synergism between knowledge of the enzymes and genes contributing to PHA synthesis in bacteria and engineering of plant metabolic pathways will be necessary for the development of crop plants that produce biodegradable plastics.
Journal ArticleDOI
Polyhydroxybutyrate, a Biodegradable Thermoplastic, Produced in Transgenic Plants
TL;DR: To produce PHB by genetic engineering in plants, genes from the bacterium Alcaligenes eutrophus that encoded the two enzymes required to convert acetoacetyl—coenzyme A to PHB were placed under transcriptional control of the cauliflower mosaic virus 35S promoter and introduced into Arabidopsis thaliana.
Journal ArticleDOI
Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris
TL;DR: Two DNA fragments containing putative control regions regulating the expression of the alcohol oxidase and dihydroxy-acetone synthase genes from the methylotrophic yeast Pichia pastoris were used in the construction of vectors for theexpression of the Escherichia coli lacZ gene.
Journal ArticleDOI
Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation
TL;DR: In contrast to earlier experiments in which expression of the PHB biosynthetic pathway in the cytoplasm led to a deleterious effect on growth, expression in plastids had no obvious effect on the growth or fertility of the transgenic plants and resulted in a 100-fold increase in the amount of PHB that accumulated.
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