Journal ArticleDOI
Test reactions for a stopped-flow apparatus:Reduction of 2,6-dichlorophenolindophenol and potassium ferricyanide by l-ascorbic acid
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TLDR
The reduction of 2,6-dichlorophenol-indophenol by l -ascorbic acid at pH 2.0 was recommended as the most practical test reaction and the reaction mechanism of the reduction was discussed.About:
This article is published in Analytical Biochemistry.The article was published on 1978-02-01. It has received 240 citations till now. The article focuses on the topics: Ascorbic acid & Ferricyanide.read more
Citations
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Study of the "molten globule" intermediate state in protein folding by a hydrophobic fluorescent probe.
Gennady V. Semisotnov,N. A. Rodionova,O. I. Razgulyaev,Vladimir N. Uversky,A. F. Gripas,R. I. Gilmanshin +5 more
TL;DR: Binding of the hydrophobia fluorescent probe, 1‐anilino‐naphthalene‐8‐sulfonate (ANS), to synthetic polypeptides and proteins with a different structural organization has been studied and it has been shown that ANS has a much stronger affinity to the protein “molten globule” state.
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Nitric oxide scavenging by red blood cell microparticles and cell-free hemoglobin as a mechanism for the red cell storage lesion.
Chenell Donadee,Nicolaas J.H. Raat,Tamir Kanias,Jesús Tejero,Janet S. Lee,Eric E. Kelley,Xuejun Zhao,Chen Liu,Hannah Reynolds,Ivan Azarov,Sheila Frizzell,E. Michael Meyer,Albert D. Donnenberg,Lirong Qu,Darrel Triulzi,Daniel B. Kim-Shapiro,Mark T. Gladwin +16 more
TL;DR: Two complementary in vivo studies suggest new mechanisms for endothelial injury and impaired vascular function associated with the most fundamental of storage lesions, hemolysis.
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Protective systems against active oxygen species in spinach: response to cold acclimation in excess light.
TL;DR: Cold acclimation led to several metabolic and biochemical changes that apparently include improved protection of the photosynthetic apparatus against active oxygen species and possible relevance for plant resistance to photoinhibition at chilling temperatures.
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Exogenous jasmonic acid can enhance tolerance of wheat seedlings to salt stress.
TL;DR: It is suggested that JA could effectively protect wheat seedlings from salt stress damage by enhancing activities of antioxidant enzymes and the concentration of antioxidative compounds to quench the excessive reactive oxygen species caused by salt stress and presented a practical implication for wheat cultivation in salt-affected soils.
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Conservation of rapid two-state folding in mesophilic, thermophilic and hyperthermophilic cold shock proteins.
Dieter Perl,Christine Welker,Thomas Schindler,Katja Schröder,Mohamed A. Marahiel,Rainer Jaenicke,Franz X. Schmid +6 more
TL;DR: This work has shown that the cold shock protein CspB from Bacillus subtilis is only marginally stable, but it folds extremely fast in a simple N \[rlharr] U two-state reaction.
References
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XLVI. On the determination of the velocity constant of a unimolecular reaction
TL;DR: In this article, the determination of the velocity constant of a unimolecular reaction is discussed. But the authors focus only on the case where the reaction can be seen as a single particle.
Journal ArticleDOI
The molar extinction coefficient of 2,6-dichlorophenol indophenol.
TL;DR: This communication deals with the pH dependence of the molar extinction coefficient of DCIP at 6oo m/z, a method for calculating this coefficient at any pH, the apparent and thermodynamic dissociation constants, and the instability of the dye at acid pH values and in alkaiine g!ycine buffers.
Journal ArticleDOI
High Speed Optical Stopped‐Flow Apparatus
TL;DR: A stopped flow apparatus for the study of fast reactions in solution is described and the measured dead time was 270 μsec with an uncertainty in the measurement of 50 μsec.
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