Journal ArticleDOI
The box C/D and H/ACA snoRNPs: key players in the modification, processing and the dynamic folding of ribosomal RNA.
TLDR
High resolution structures of the archaeal C/D and H/ACA sRNPs have not only provided a detailed understanding of the molecular architecture of these complexes but also produced key insights into substrate binding and product release.Abstract:
Box C/D and H/ACA RNPs are essential ribonucleoprotein particles that are found throughout both eukaryotes [small nucleolar RNPs (snoRNPs)] and archaea [snoRNP-like complexes (sRNPs)]. These complexes catalyze the site-specific pseudouridylation and most of the methylation of ribosomal RNA (rRNA). The numerous modifications, which are clustered in functionally important regions of the rRNA, are important for rRNA folding and ribosome function. The RNA component of the complexes [small nucleolar RNA (snoRNA) or small RNA (sRNA)] functions in substrate binding by base pairing with the target site and as a scaffold coordinating the organization of the complex. In eukaryotes, a subset of snoRNPs do not catalyze modification but, through base pairing to the rRNA or flanking precursor sequences, direct pre-rRNA folding and are essential for rRNA processing. In the last few years there have been significant advances in our understanding of the structure of archaeal sRNPs. High resolution structures of the archaeal C/D and H/ACA sRNPs have not only provided a detailed understanding of the molecular architecture of these complexes but also produced key insights into substrate binding and product release. In both cases, this is mediated by significant movement in the complexes. Advances have also been made in our knowledge of snoRNP recruitment and release from pre-ribosome complexes in eukaryotes. New snoRNA-rRNA interactions have been documented, and the roles of RNA helicases in releasing snoRNP complexes from the rRNA have been described.read more
Citations
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Journal ArticleDOI
The noncoding RNA revolution-trashing old rules to forge new ones.
TL;DR: The pathway of ncRNA research is described, where every established "rule" seems destined to be overturned.
Journal ArticleDOI
Dynamic RNA Modifications in Gene Expression Regulation
TL;DR: Roles for mRNA modification in nearly every aspect of the mRNA life cycle, as well as in various cellular, developmental, and disease processes are revealed.
Journal ArticleDOI
A census of human RNA-binding proteins.
TL;DR: This work presents a census of 1,542 manually curated RBPs that are analysed for their interactions with different classes of RNA, their evolutionary conservation, their abundance and their tissue-specific expression, a critical step towards the comprehensive characterization of proteins involved in human RNA metabolism.
Journal ArticleDOI
Ribosome Biogenesis in the Yeast Saccharomyces cerevisiae
TL;DR: Yeast ribosome biogenesis provide useful models for ribosomopathies, diseases in humans that result from failure to properly assemble ribosomes.
Journal ArticleDOI
An overview of pre‐ribosomal RNA processing in eukaryotes
Anthony K. Henras,Célia Plisson-Chastang,Marie-Françoise O'Donohue,Anirban Chakraborty,Pierre-Emmanuel Gleizes +4 more
TL;DR: A consensus scheme of pre‐ribosomal RNA maturation is emerging from studies in various kinds of eukaryotic organisms, including small nucleolar ribonucleoparticles, but major differences between mammalian and yeast pre-ribosome RNA processing have recently come to light.
References
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A Human snoRNA with MicroRNA-Like Functions
Christine Ender,Azra Krek,Azra Krek,Marc R. Friedländer,Michaela Beitzinger,Lasse Weinmann,Wei Chen,Sébastien Pfeffer,Nikolaus Rajewsky,Gunter Meister +9 more
TL;DR: A class of small RNAs in human cells that originate from snoRNAs and can function like miRNAs is identified, suggesting a role for ACA45-processing products in posttranscriptional gene silencing.
Journal ArticleDOI
Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs.
Zsuzsanna Kiss-László,Yves Henry,Jean-Pierre Bachellerie,Michèle Caizergues-Ferrer,Tamás Kiss +4 more
TL;DR: The conclusion that sno-RNAs function in covalent modification of the sugar moieties of ribonucleotides demonstrates that eukaryotic small nuclear RNAs have a more versatile cellular function than earlier anticipated.
Journal ArticleDOI
The snoRNA HBII-52 Regulates Alternative Splicing of the Serotonin Receptor 2C
Shivendra Kishore,Stefan Stamm +1 more
TL;DR: The results show that a snoRNA regulates the processing of an mRNA expressed from a gene located on a different chromosome, and the results indicate that a defect in pre-mRNA processing contributes to the Prader-Willi syndrome.
Journal ArticleDOI
Site-specific pseudouridine formation in preribosomal RNA is guided by small nucleolar RNAs.
TL;DR: It is demonstrated that site-specific pseudouridylation of rRNAs relies on short ribosomal signal sequences that are complementary to sequences in box H/ACA snoRNAs.