Journal ArticleDOI
The C2 Domain of PKCα Is a Ca2+-dependent PtdIns(4,5)P2 Sensing Domain: A New Insight into an Old Pathway
Sonia Sánchez-Bautista,Consuelo Marín-Vicente,Juan C. Gómez-Fernández,Senena Corbalán-García +3 more
TLDR
It was shown that although a fraction of PtdIns(4,5)P2 is hydrolyzed to generate diacylglycerol and IP3, an important amount still remains in the membrane where it is available to activate PKCalpha, requiring revision of the currently accepted model of PK Calpha recruitment to the membrane and its activation.About:
This article is published in Journal of Molecular Biology.The article was published on 2006-10-06. It has received 59 citations till now. The article focuses on the topics: C2 domain & Diacylglycerol kinase.read more
Citations
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Journal ArticleDOI
Membrane recognition by phospholipid-binding domains.
TL;DR: Many different globular domains bind to the surfaces of cellular membranes, or to specific phospholipid components in these membranes, and this binding is often tightly regulated.
Journal ArticleDOI
Survey of the year 2005: literature on applications of isothermal titration calorimetry.
TL;DR: An overview of the literature for 2005 is attempted to highlight works of interest and novelty and draw attention to those works which it is felt have provided a route to better analysis and increased the ability to understand the meaning of thermodynamic change on binding.
Journal ArticleDOI
Signaling through C2 domains: more than one lipid target.
TL;DR: This review summarizes the main structural and functional findings on Ca(2+) and lipid interactions by C2 domains, including the discovery of the phosphoinositide-binding site located in the β3-β4 strands.
Journal ArticleDOI
Protein kinase C: perfectly balanced.
TL;DR: The tightly controlled mechanisms that ensure PKC activity is perfectly balanced are described and what happens when these controls are deregulated are described.
Journal ArticleDOI
Conditional peripheral membrane proteins: Facing up to limited specificity
TL;DR: A broader range of recognition modes presents significant methodological challenges for a full structural understanding of regulated relocalization of signaling and trafficking proteins.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Book
Principles of fluorescence spectroscopy
TL;DR: This book describes the fundamental aspects of fluorescence, the biochemical applications of this methodology, and the instrumentation used in fluorescence spectroscopy.
Journal ArticleDOI
Protein kinase C and lipid signaling for sustained cellular responses.
TL;DR: It is now becoming evident that stimulation of a cell surface receptor initiates a degradation cascade of various membrane lipid constituents that has potentials to induce, intensify, and prolong the activation of protein kinase C that is needed for sustained cellular responses.
Journal ArticleDOI
Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells.
TL;DR: Fluorescence resonance energy transfer measurements in living cells revealed that acyl but not prenyl modifications promote clustering in lipid rafts, and the nature of the lipid anchor on a protein is sufficient to determine submicroscopic localization within the plasma membrane.