Journal ArticleDOI
The chemistry and biology of unusual DNA structures adopted by oligopurine.oligopyrimidine sequences.
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TLDR
A family of unusual DNA structures has been discovered in segments with predominantly purines in one strand (pur · pyr sequences) that are overrepresented in eukaryotic DNA and have been mapped near genes and recombination hot spots.Abstract:
A family of unusual DNA structures has been discovered in segments with predominantly purines in one strand (pur.pyr sequences). These sequences are overrepresented in eukaryotic DNA and have been mapped near genes and recombination hot spots. When cloned into recombinant plasmids, many pur.pyr sequences are reactive to chemical and enzymic probes that are generally specific for single-stranded DNA. An intramolecular triplex is adopted by mirror repeats of G's and A's. Other non-B DNA structures adopted by similar sequences remain to be fully clarified but may be a family of related conformations. It is likely that these unorthodox structures play an important role in the function of the eukaryotic genome.read more
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References
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Journal ArticleDOI
Sequence-specific cleavage of double helical DNA by triple helix formation
Heinz E. Moser,Peter B. Dervan +1 more
TL;DR: The location and asymmetry of the cleavage pattern reveal that the homopyrimidine-EDTA probes bind in the major groove parallel to thehomopurine strand of Watson-Crick double helical DNA.
Journal ArticleDOI
DNA bending at adenine . thymine tracts.
TL;DR: Intrinsic bending of DNA molecules results from local structural polymorphism in regions of homopolymeric dA · dT which are at least 4 base pairs long; the A · T tracts must be repeated in phase with the helix screw.
Journal ArticleDOI
The structure of an oligo(dA)·oligo(dT) tract and its biological implications
TL;DR: The crystal structure of a B-type DNA dodecamer containing a homopolymeric run of six A·T base pairs shows that this region possesses special structural features, including a system of bifurcated hydrogen bonds, which explains some of the properties of this simple homopolymer.
Journal ArticleDOI
Site-specific oligonucleotide binding represses transcription of the human c-myc gene in vitro
TL;DR: It is discussed that triplex formation (site-specific RNA binding to a DNA duplex) could serve as the basis for an alternative program of gene control in vivo.
Journal ArticleDOI
Telomeric DNA oligonucleotides form novel intramolecular structures containing guanine-guanine base pairs
TL;DR: Detailed analysis of the hairpin structure formed by the telomeric sequence from Tetrahymena, (T2G4)4, shows that it is a unique structure stabilized by hydrogen bonds and contains G residues in the syn conformation, and it is proposed that this novel form of DNA is important for telomere function and sets a precedent for the biological relevance of non-Watson-Crick base-paired DNA structures.
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Sequence-specific cleavage of double helical DNA by triple helix formation
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