Journal ArticleDOI
The cytoskeletal framework of sea urchin eggs and embryos: developmental changes in the association of messenger RNA.
Randall T. Moon,Randall T. Moon,Roberto F. Nicosia,Roberto F. Nicosia,Cherie L. Olsen,Cherie L. Olsen,Merrill B. Hille,William R. Jeffery,William R. Jeffery +8 more
TLDR
Results indicate a correlation between the activation of protein synthesis in eggs and the association of polyribosomes with the CSK, and several lines of evidence suggest that mRNA is a component of theCSK of both eggs and embryos.About:
This article is published in Developmental Biology.The article was published on 1983-02-01. It has received 79 citations till now. The article focuses on the topics: Polysome & Protein biosynthesis.read more
Citations
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Quantitative analysis of in situ hybridization methods for the detection of actin gene expression.
TL;DR: An efficient, quantitative approach for the optimization of in situ hybridization using double-stranded recombinant DNA probes, which is simpler and less destructive to cellular RNA and morphology than other protocols.
Journal ArticleDOI
Changes in actin distribution during fertilization of the mouse egg
TL;DR: Unfertilized mouse oocytes and eggs 1-8 h after fertilization in vitro were examined at the light microscope level for structural changes, distribution of actin, surface binding of concanavalin A (Con A) and chromosomal distribution and condensation.
Journal ArticleDOI
Requirement of microfilaments in sorting of actin messenger RNA
TL;DR: It is suggested that actin-binding proteins may participate in mRNA sorting in chicken embryo fibroblasts.
Journal ArticleDOI
Diversity of cytoplasmic functions for the 3' untranslated region of eukaryotic transcripts
Carolyn J. Decker,Roy Parker +1 more
TL;DR: The 3' untranslated region (3' UTR) can control gene expression by affecting the localization, stability and translation of mRNAs through a direct or indirect interaction between the 3' and 5' ends of m RNAs.
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Emerging role for the cytoskeleton as an organizer and regulator of translation
Seyun Kim,Pierre A. Coulombe +1 more
TL;DR: Evidence favours the hypothesis that the cytoskeleton participates in the spatial organization and regulation of translation, at both the global and local level, in a manner that is crucial for cellular growth, proliferation and function.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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Ribonucleic acid isolated by cesium chloride centrifugation.
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RNA metabolism in the HeLa cell nucleus.
TL;DR: Experiments in which radioactive label is chased shows that there is no high molecular weight RNA permanently associated with the nucleus, and all of the early precursors to cytoplasmic ribosomal RNA are contained in the nuclear fraction.