The effect of ascorbic acid on the nature and production of collagen and elastin by rat smooth-muscle cells.
Y A de Clerck,Peter A. Jones +1 more
TLDR
The appearance of insoluble collagen in the extracellular matrices produced by cultured human fibroblasts and calf endothelial cells was completely dependent on the presence of ascorbic acid and had variable effects on the quantity of glycoprotein(s) present in the matrix.Abstract:
1 The effects of various concentrations of ascorbic acid on the quality and quantity of the insoluble extracellular matrices produced by two strains of cultured rat smooth-muscle cells were studied 2 Ascorbic acid was necessary for the appearance of insoluble collagen in the extracellular matrix 3 Secretion of soluble collagen continued in the absence of ascorbic acid, but this soluble collagen was markedly underhydroxylated 4 The amount of insoluble collagen present in the matrix was directly related to the ascorbic acid concentration 5 The insoluble collagen that appeared in the matrix under conditions where ascorbic acid was limiting was no more than 7% underhydroxylated 6 In contrast, the amount of insoluble elastin produced was inversely proportional to the ascorbic acid concentration 7 The elastin produced in the absence of ascorbic acid had the expected amino acid composition, but hydroxyproline was absent 8 The hydroxyproline content of elastin was also directly dependent on the ascorbic acid concentration 9 Ascorbic acid had variable effects on the quantity of glycoprotein(s) present in the matrix 10 The appearance of insoluble collagen in the extracellular matrices produced by cultured human fibroblasts and calf endothelial cells was also completely dependent on the presence of ascorbic acidread more
Citations
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The Biochemical Functions of Ascorbic Acid
Sasha Englard,Sam Seifter +1 more
TL;DR: A review of the history and present situation of drug abuse in the United States, as well as some of the aspects of drug rehabilitation that have changed over the years, is provided.
Journal ArticleDOI
Regulation of collagen synthesis by ascorbic acid.
TL;DR: The results suggest that collage polypeptide synthesis, posttranslational hydroxylations, and activities of the twohydroxylases are independently regulated by ascorbate.
Journal ArticleDOI
Differentiation of axon-related Schwann cells in vitro. I. Ascorbic acid regulates basal lamina assembly and myelin formation
TL;DR: It is suggested that ascorbic acid promotes Schwanncell myelin formation by enabling the Schwann cell to assemble a basal lamina, which is required for complete differentiation.
Journal ArticleDOI
Degradation of connective tissue matrices by macrophages. I. Proteolysis of elastin, glycoproteins, and collagen by proteinases isolated from macrophages.
TL;DR: The data indicate that macrophages at inflammatory sites contain and secrete proteolytic enzymes that could degrade the extracellular matrix, and human granulocyte elastase effectively degraded the matrix glycoproteins, elastin, and, to a lesser extent, collagens.
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Destruction of Extracellular Matrices Containing Glycoproteins, Elastin, and Collagen by Metastatic Human Tumor Cells
Peter A. Jones,Yves A. DeClerck +1 more
TL;DR: These quantitative studies, in which tumor cells were grown in contact with a complex extracellular matrix possessing some of the characteristics of connective tissue, should have a bearing on tumor cell invasion.
References
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Kale Juva,Darwin J. Prockop +1 more
TL;DR: Although the present procedure measures total radioactive hydroxyproline rather than the specific radioactivity of hydroxy Proline, it is more readily applicable to samples from a variety of sources.
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Aggregation of Cartilage Proteoglycans: III. CHARACTERISTICS OF THE PROTEINS ISOLATED FROM TRYPSIN DIGESTS OF AGGREGATES
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