Journal ArticleDOI
The effects of “Cell age” upon the lethal effects of physical and chemical mutagens in the yeast, Saccharomyces cerevisiae
TLDR
The results indicated that the differential uptake of the chemical mutagens was responsible for at least a fraction of the variations in cell sensitivity observed in yeast cultures at different phases of growth.Abstract:
Summary Yeast cultures progressing from the exponential to the stationary phase of growth showed changes in cell sensitivity to physical agents such as UV light, heat shock at 52° C and the chemical mutagens ethyl methane sulphonate, nitrous acid and mitomycin C. Exponential phase cells showed maximum resistance to UV light and minimum resistance to heat shock and the three chemicals. The increased resistance of exponential phase cells to UV light was shown to be dependent upon the functional integrity of the RAD
50 gene. Treatment of growing yeast cultures with radioactively labelled ethyl methane sulphonate indicated the preferential uptake of radioactivity during the sensitive exponential stage of growth. The results indicated that the differential uptake of the chemical mutagens was responsible for at least a fraction of the variations in cell sensitivity observed in yeast cultures at different phases of growth.read more
Citations
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Journal ArticleDOI
Rapid and preferential activation of the c-jun gene during the mammalian UV response.
TL;DR: It is found that expression of both c-jun and c-fos, which encode proteins that participate in formation of the AP-1 complex, is rapidly induced by two different DNA-damaging agents: UV and H2O2.
Book ChapterDOI
Physiology of osmotolerance in fungi
Anders Blomberg,Lennart Adler +1 more
TL;DR: Combined genetic and physiological analysis is required for a deeper understanding of fungus-water relations and has revealed sequential induction of osmotically controlled genes in enteric bacteria and given exciting insights in signal transduction and regulation of the process.
Journal ArticleDOI
Roles of glycerol and glycerol-3-phosphate dehydrogenase (NAD+) in acquired osmotolerance of Saccharomyces cerevisiae.
Anders Blomberg,Lennart Adler +1 more
TL;DR: It is shown that protein synthesis was required to establish the osmotolerance state in Saccharomyces cerevisiae, and the increased glycerol accumulation was shown to be not merely a result of enhanced production rate but also of increased retention of Glycerol.
Journal ArticleDOI
Deoxyribonucleoside triphosphate levels: A critical factor in the maintenance of genetic stability
Bernard A. Kunz,Susanne E. Kohalmi,Thomas A. Kunkel,Christopher K. Mathews,Evan M. McIntosh,John A. Reidy +5 more
TL;DR: In this article, the biochemical and genetic mechanisms responsible for regulating the production and relative amounts of intracellular DNA precursors, describe the many outcomes of perturbations in DNA precursor levels, and discuss implications of such imbalances for sensitivity to DNA-damaging agents, population monitoring, and human diseases.
Journal ArticleDOI
Decreased susceptibility of melanized Cryptococcus neoformans to UV light.
Yulin Wang,Arturo Casadevall +1 more
TL;DR: Melanized Cryptococcus neoformans cells were less susceptible than nonmelanized cells to the fungicidal effects of UV light and Phenoloxidase-catalyzed production of melanin-like pigments may protect the fungus against ionizing radiation.
References
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Journal ArticleDOI
Changes in x-ray sensitivity of HeLa cells during the division cycle.
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Journal ArticleDOI
Hydroxyurea: Differential Lethal Effects on Cultured Mammalian Cells during the Cell Cycle
TL;DR: Hydroxyurea has a differential lethal effect on cultured Chinesehamster cells that are at different stages in their cell cycle, which may be useful for synchronizing asynchronous cell populations and explaining effects of the drug in human therapy.
Journal ArticleDOI
The isolation, genetics and survival characteristics of ultraviolet light-sensitive mutants in yeast
B.S. Cox,J.M. Parry +1 more
TL;DR: 96 mutants of yeast have been isolated which were more sensitive than wild-type to ultraviolet irradiation, and genetic analysis showed that many are inherited as single mendelian recessive mutations, and that they occupy at lest 22 different loci.