Journal ArticleDOI
The lipid requirement of the (Ca2+ + Mg2+)-ATPase in the human erythrocyte membrane, as studied by various highly purified phospholipases.
B. Roelofsen,H.J. Schatzmann +1 more
TLDR
It is concluded that only the glycerophospholipids in the human erythrocyte membrane are involved in the maintenance of the (Ca2+ + Mg2+)-ATPase activity, and in particular that fraction of these phospholipid fraction located in the inner half of the membrane.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1977-01-04. It has received 77 citations till now. The article focuses on the topics: Phospholipase & ATPase.read more
Citations
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Journal ArticleDOI
Lipid polymorphism and the functional roles of lipids in biological membranes.
Pieter R. Cullis,B. de Kruijff +1 more
TL;DR: The possible functional roles of lipids are reviewed in terms of previous models such as the fluid mosaic model of Singer and Nicolson or the earlier unit membrane model so that the requirement for an alternative approach becomes apparent.
Journal ArticleDOI
Interactions between components in biological membranes and their implications for membrane function.
Gheorghe Benga,Ross P. Holmes +1 more
Journal ArticleDOI
Calcium in human red blood cells.
H. J. Schatzmann,H. Bürgin +1 more
TL;DR: Red blood cells from adult humans are able to keep the Ca concentration in the cytosol several orders of magnitude below that of blood plasma, indicating that the Ca2+ concentration may be as low as M or less in the normal state of the cell (Cao2+ = M).
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Chromatographically homogeneous lecithin from egg phospholipids.
TL;DR: The modified method involved application of crude egg phosphatides to a column of alumina in the proportion of 1 g phosphatide/25 g alumina, and elution of the lecithin fraction with the 2-component solvent system chloroform:methanol, 9:1 by vol.
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The asymmetric distribution of phospholipids in the human red cell membrane. A combined study using phospholipases and freeze-etch electron microscopy
TL;DR: It is concluded that thisospholipid fraction (which contains the majority of the choline-containing phospholipids and some phosphatidylethanolamine) forms the outer monolayer of the membrane.
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A rapid and sensitive sub-micro phosphorus determination
Journal ArticleDOI
Phospholipid model membranes. I. Structural characteristics of hydrated liquid crystals.
TL;DR: The size and shape of the particles as well as the degree of birefringence and the extent to which negative stain penetrates between the lamellae during electron microscopy were characteristic of the various phospholipid species used.