The m6A RNA Modification Quantity and mRNA Expression Level of RNA Methylation-Related Genes in Head and Neck Squamous Cell Carcinoma Cell Lines and Patients.
Kamila Romanowska,Agnieszka Anna Rawłuszko-Wieczorek,Łukasz Marczak,Agnieszka Kosińska,Wiktoria Maria Suchorska,Wojciech Golusiński +5 more
- Vol. 11, Iss: 6, pp 908
TLDR
In this paper, the m6A modification at the nitrogen sixth of adenosine (m6A, N6-methyladenosine) is the most abundant RNA modification which plays a crucial role in all RNA metabolic aspects.Abstract:
RNA methylation at the nitrogen sixth of adenosine (m6A, N6-methyladenosine) is the most abundant RNA modification which plays a crucial role in all RNA metabolic aspects. Recently, m6A modification has been assigned to mediate the biological processes of cancer cells, but their significance in HNSCC development is still poorly described. Thus, the main aim of this study was to globally quantify m6A modification by the mass spectrometry approach and determine the mRNA expression level of selected m6A RNA methyltransferase (METTL3), demethylase (FTO), and m6A readers (YTHDF2, YTHDC2) in 45 HNSCC patients and 4 cell lines (FaDu, Detroit 562, A-253 and SCC-15) using qPCR. In the results, we have not observed differences in the global amount of m6A modification and the mRNA level of the selected genes between the cancerous and paired-matched histopathologically unchanged tissues from 45 HNSCC patients. However, we have found a positive correlation between selected RNA methylation machinery genes expression and m6A abundance on total RNA and characterized the transcript level of those genes in the HNSCC cell lines. Moreover, the lack of global m6A differences between cancerous and histopathologically unchanged tissues suggests that m6A alterations in specific RNA sites may specifically influence HNSCC tumorigenesis.read more
Citations
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The RNA N6-Methyladenosine Demethylase FTO Promotes Head and Neck Squamous Cell Carcinoma Proliferation and Migration by Increasing CTNNB1.
TL;DR: In this article, the role of RNA N6-methyladenosine demethylase fat mass and obesityassociated protein (FTO) in head and neck squamous cell carcinoma (HNSCC) was investigated.
Journal ArticleDOI
The m6A RNA Modification Quantity and the Prognostic Effect of Reader YTHDC2 in Colorectal Cancer
Tianyu Liu,Wentao Tang,Yijiao Chen,Yu Liu,Dong Xu,Yudong Jiang,Shizhao Zhou,Xiaorui Qin,Lei Ren,Wenju Chang,Jianmin Xu +10 more
TL;DR: It is suggested that the global level of m6A did not change in CRC and identified that lower YTHDC2 as a prognostic marker for worse survival of CRC.
Journal ArticleDOI
Genetics and Molecular Biology of Head and Neck Cancer.
TL;DR: Ahead and neck cancer (HNC) is a multistep process proceeding from single gene mutations generated by carcinogens to the substantial dysregulation of metabolic processes, as described in this article.
References
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Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq
Dan Dominissini,Sharon Moshitch-Moshkovitz,Schraga Schwartz,Schraga Schwartz,Mali Salmon-Divon,Lior Ungar,Sivan Osenberg,Sivan Osenberg,Karen Cesarkas,Jasmine Jacob-Hirsch,Ninette Amariglio,Martin Kupiec,Rotem Sorek,Gideon Rechavi,Gideon Rechavi +14 more
TL;DR: The findings suggest that RNA decoration by m6A has a fundamental role in regulation of gene expression, and a subset of stimulus-dependent, dynamically modulated sites is identified.
Journal ArticleDOI
Where, When, and How: Context-Dependent Functions of RNA Methylation Writers, Readers, and Erasers
TL;DR: This review highlights recent progress in understanding the function of N6-methyladenosine (m6A), the most abundant internal mark on eukaryotic mRNA, in light of the specific biological contexts of m6A effectors, and emphasizes the importance of context for RNA modification regulation and function.
Journal ArticleDOI
Dynamic transcriptomic m6A decoration: writers, erasers, readers and functions in RNA metabolism.
TL;DR: The current understanding of the m6A modification, particularly the functions of its writers, erasers, readers in RNA metabolism, is described, with an emphasis on its role in regulating the isoform dosage of mRNAs.
Journal ArticleDOI
Head and Neck Squamous Cell Carcinoma: Update on Epidemiology, Diagnosis, and Treatment.
TL;DR: The changing epidemiology, advances in diagnosis, and treatment options for squamous cell cancers of the head and neck, along with data on risk stratification specific to oropharyngeal cancer are focused on, and the direction of current trials is highlighted.
Journal ArticleDOI
A majority of m6A residues are in the last exons, allowing the potential for 3′ UTR regulation
Shengdong Ke,Endalkachew Ashenafi Alemu,Claudia Mertens,Emily Conn Gantman,John J. Fak,John J. Fak,Aldo Mele,Aldo Mele,Bhagwattie Haripal,Ilana Zucker-Scharff,Ilana Zucker-Scharff,Michael Moore,Michael Moore,Christopher Y. Park,Cathrine Broberg Vågbø,Anna Kusśnierczyk,Arne Klungland,Arne Klungland,James E. Darnell,Robert B. Darnell +19 more
TL;DR: It is found that m(6)A density peaks early in the 3' UTR and that, among transcripts with alternative polyA (APA) usage in both the brain and the liver, brain transcripts preferentially use distal polyA sites, as reported, and also show higher proximal m( 6)Adensity in the last exons.